Repeated-batch production of omega-3 enriched biomass of Chlorella sorokiniana via calcium-induced homeoviscous adaptation
- Authors
- Shim, Sung Jin; Hong, Min Eui; Chang, Won Seok; Sim, Sang Jun
- Issue Date
- 5월-2020
- Publisher
- ELSEVIER SCI LTD
- Keywords
- Ca2+-based 1-stage recycling strategy; Ca2+-induced homeoviscous adaptation; Chlorella sorokiniana; Eicosapentaenoic acid (EPA); Omega-3 fatty acids
- Citation
- BIORESOURCE TECHNOLOGY, v.303
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIORESOURCE TECHNOLOGY
- Volume
- 303
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/56107
- DOI
- 10.1016/j.biortech.2020.122944
- ISSN
- 0960-8524
- Abstract
- This study aimed to improve valuable omega-3 fatty acids production in freshwater microalgae at normal temperature by inducing homeoviscous adaptation using CaCl2, which could have a role in decreasing the cellular membrane fluidity followed by increasing the rigidity of cell wall and membranes. At 10 mM CaCl2, simultaneous biomass and lipid production was obtained by Ca2+-based single strategy without considerable sacrifice of cellular logarithmic growth in Chlorella sorokiniana. The cells cultured at 10 mM CaCl2 (1-stage) showed relatively high levels of cellular membrane fluidity, caused by increased content in unsaturated fatty acids, compared to the conventional culture strategy (2-stage). Moreover, when this process was recycled by repeated-batch fermentation, the EPA productivity of 1-stage was 4.338 mg L-1 d(-1), conspicuously increased by over 1300% compared to 2-stage. This strategy enhances the valuable omega-3 production, which can be commercially used for mass cultivation of omega-3-enriched biomass in the microalgae industry.
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Collections - College of Engineering > Department of Chemical and Biological Engineering > 1. Journal Articles
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