Phylogenetic Analysis and In Vitro Bifunctional Nuclease Assay of Arabidopsis BBD1 and BBD2
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Huque, A. K. M. Mahmudul | - |
dc.contributor.author | So, Won Mi | - |
dc.contributor.author | You, Min Kyoung | - |
dc.contributor.author | Shin, Jeong Sheop | - |
dc.date.accessioned | 2021-08-31T01:35:21Z | - |
dc.date.available | 2021-08-31T01:35:21Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2020-05 | - |
dc.identifier.issn | 1420-3049 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/56164 | - |
dc.description.abstract | Nucleases are a very diverse group of enzymes that play important roles in many crucial physiological processes in plants. We previously reported that the highly conserved region (HCR), domain of unknown function 151 (DUF151) and UV responsive (UVR) domain-containing OmBBD is a novel nuclease that does not share homology with other well-studied plant nucleases. Here, we report that DUF151 domain-containing proteins are present in bacteria, archaea and only Viridiplantae kingdom of eukarya, but not in any other eukaryotes. Two Arabidopsis homologs of OmBBD, AtBBD1 and AtBBD2, shared 43.69% and 44.38% sequence identity and contained all three distinct domains of OmBBD. We confirmed that the recombinant MBP-AtBBD1 and MBP-AtBBD2 exhibited non-substrate-specific DNase and RNase activity, like OmBBD. We also found that a metal cofactor is not necessarily required for DNase activity of AtBBD1 and AtBBD2, but their activities were much enhanced in the presence of Mg2+ or Mn2+. Using a yeast two-hybrid assay, we found that AtBBD1 and AtBBD2 each form a homodimer but not a heterodimer and that the HCR domain is possibly crucial for dimerization. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.subject | PROGRAMMED CELL-DEATH | - |
dc.subject | CA2+-DEPENDENT NUCLEASE | - |
dc.subject | DNA FRAGMENTATION | - |
dc.subject | MOLECULAR-CLONING | - |
dc.subject | SENESCENCE | - |
dc.subject | LEAF | - |
dc.subject | IDENTIFICATION | - |
dc.subject | DEGRADATION | - |
dc.subject | EXPRESSION | - |
dc.subject | GENE | - |
dc.title | Phylogenetic Analysis and In Vitro Bifunctional Nuclease Assay of Arabidopsis BBD1 and BBD2 | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Shin, Jeong Sheop | - |
dc.identifier.doi | 10.3390/molecules25092169 | - |
dc.identifier.scopusid | 2-s2.0-85084721752 | - |
dc.identifier.wosid | 000535695900165 | - |
dc.identifier.bibliographicCitation | MOLECULES, v.25, no.9 | - |
dc.relation.isPartOf | MOLECULES | - |
dc.citation.title | MOLECULES | - |
dc.citation.volume | 25 | - |
dc.citation.number | 9 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.subject.keywordPlus | PROGRAMMED CELL-DEATH | - |
dc.subject.keywordPlus | CA2+-DEPENDENT NUCLEASE | - |
dc.subject.keywordPlus | DNA FRAGMENTATION | - |
dc.subject.keywordPlus | MOLECULAR-CLONING | - |
dc.subject.keywordPlus | SENESCENCE | - |
dc.subject.keywordPlus | LEAF | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | DEGRADATION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordAuthor | Arabidopsis | - |
dc.subject.keywordAuthor | DUF151 | - |
dc.subject.keywordAuthor | nuclease | - |
dc.subject.keywordAuthor | OmBBD | - |
dc.subject.keywordAuthor | AtBBD1 | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.