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Enzymatic Synthesis of Anabolic Steroid Glycosides by Glucosyltransferase from Terribacillus sp. PAMC 23288

Authors
Yu, Eun-JiYamaguchi, TokutaroLee, Joo-HoLim, A-RangLee, Jun HyuckPark, HyunOh, Tae-Jin
Issue Date
4월-2020
Publisher
KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
Keywords
Testosterone; nandrolone; glucosylation; neuroprotective activity; rotenone-induced apoptosis; steroid
Citation
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.30, no.4, pp.604 - 614
Indexed
SCIE
SCOPUS
KCI
Journal Title
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
Volume
30
Number
4
Start Page
604
End Page
614
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/56765
DOI
10.4014/jmb.1911.11057
ISSN
1017-7825
Abstract
The application of steroids has steadily increased thanks to their therapeutic effects. However, alternatives are required due their severe side effects; thus, studies on the activities of steroid derivatives are underway. Sugar derivatives of nandrolone, which is used to treat breast cancer, as well as cortisone and prednisone, which reduce inflammation, pain, and edema, are unknown. We linked O-glucose to nandrolone and testosterone using UDP-glucosyltransferase (UGT-1) and, then, tested their bioactivities in vitro. Analysis by NMR showed that the derivatives were 17 beta-nandrolone beta-D-glucose and 17 beta-testosterone beta-D-glucose, respectively. The viability was higher and cytotoxicity was evident in PC12 cells incubated with rotenone and, testosterone derivatives, compared to the controls. SH-SY5Y cells incubated with H2O2 and nandrolone derivatives remained viable and cytotoxicity was attenuated. Both derivatives enhanced neuronal protective effects and increased the amounts of cellular ATP.
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