Mesenchymal stem cells reduce ER stress via PERK-Nrf2 pathway in an aged mouse model
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Eun Joo | - |
dc.contributor.author | Cardenes, Nayra | - |
dc.contributor.author | Alvarez, Diana | - |
dc.contributor.author | Sellares, Jacobo | - |
dc.contributor.author | Sembrat, John | - |
dc.contributor.author | Aranda, Paola | - |
dc.contributor.author | Peng, Yating | - |
dc.contributor.author | Bullock, Jordan | - |
dc.contributor.author | Nouraie, Seyed M. | - |
dc.contributor.author | Mora, Ana L. | - |
dc.contributor.author | Rojas, Mauricio | - |
dc.date.accessioned | 2021-08-31T04:48:56Z | - |
dc.date.available | 2021-08-31T04:48:56Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2020-04 | - |
dc.identifier.issn | 1323-7799 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/56782 | - |
dc.description.abstract | Background and objective Mesenchymal stem cells (MSC) have been shown to ameliorate the deleterious effects of bleomycin in murine models. However, the mechanism responsible for protection from pulmonary fibrosis by stem cell therapy is still poorly understood, especially in terms of endoplasmic reticulum (ER) stress. We hypothesized that during bleomycin-induced lung injury, markers of ER stress, specifically the activation of the unfolded protein response (UPR), increase during injury, resembling the kinetics of collagen deposition in the lung described for the bleomycin model. We aimed to elucidate the possible role of MSC in ER stress modulation. Methods To determine the kinetics of ER stress in aged mice, the expression of ER stress markers after bleomycin lung injury was measured in old mice at different time points (days 0, 3, 7, 14 and 21). To evaluate the consequences of systemic delivery of MSC on lung ER stress in the bleomycin model, we evaluated changes in body weight, lung histology and protein expression of ER stress markers. Results The level of expression of UPR transcription factor XBP-1 and its regulator BiP was elevated at day 7 and progressively increased up to day 21. MSC inhibited BiP expression in bleomycin-induced ER stress, attenuating ER stress via the protein kinase RNA-like ER kinase (PERK)-Nrf2 pathway. The expression levels of other ER stress markers were not perturbed by MSC. Conclusion Our data suggest that MSC operate on ER stress via several pathways, but the PERK-Nrf2 pathway revealed to be the main functioning pathway in our bleomycin model. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.subject | ENDOPLASMIC-RETICULUM STRESS | - |
dc.subject | IDIOPATHIC PULMONARY-FIBROSIS | - |
dc.subject | LUNG INJURY | - |
dc.subject | BLEOMYCIN | - |
dc.subject | PATHOGENESIS | - |
dc.subject | DEFICIENCY | - |
dc.subject | OLD | - |
dc.title | Mesenchymal stem cells reduce ER stress via PERK-Nrf2 pathway in an aged mouse model | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Eun Joo | - |
dc.identifier.doi | 10.1111/resp.13646 | - |
dc.identifier.scopusid | 2-s2.0-85082095554 | - |
dc.identifier.wosid | 000479624900001 | - |
dc.identifier.bibliographicCitation | RESPIROLOGY, v.25, no.4, pp.417 - 426 | - |
dc.relation.isPartOf | RESPIROLOGY | - |
dc.citation.title | RESPIROLOGY | - |
dc.citation.volume | 25 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 417 | - |
dc.citation.endPage | 426 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Respiratory System | - |
dc.relation.journalWebOfScienceCategory | Respiratory System | - |
dc.subject.keywordPlus | ENDOPLASMIC-RETICULUM STRESS | - |
dc.subject.keywordPlus | IDIOPATHIC PULMONARY-FIBROSIS | - |
dc.subject.keywordPlus | LUNG INJURY | - |
dc.subject.keywordPlus | BLEOMYCIN | - |
dc.subject.keywordPlus | PATHOGENESIS | - |
dc.subject.keywordPlus | DEFICIENCY | - |
dc.subject.keywordPlus | OLD | - |
dc.subject.keywordAuthor | ageing | - |
dc.subject.keywordAuthor | cell therapy | - |
dc.subject.keywordAuthor | endoplasmic reticulum stress | - |
dc.subject.keywordAuthor | lung fibrosis | - |
dc.subject.keywordAuthor | mesenchymal stem cell | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.