Development of multi-well-based electrochemical dissolved oxygen sensor array
DC Field | Value | Language |
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dc.contributor.author | Han, Ji-Hoon | - |
dc.contributor.author | Kim, Saeyoung | - |
dc.contributor.author | Choi, Jaesung | - |
dc.contributor.author | Kang, Sora | - |
dc.contributor.author | Pak, Youngmi Kim | - |
dc.contributor.author | Pak, James Jungho | - |
dc.date.accessioned | 2021-08-31T08:28:57Z | - |
dc.date.available | 2021-08-31T08:28:57Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2020-03-01 | - |
dc.identifier.issn | 0925-4005 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/57359 | - |
dc.description.abstract | This paper describes a multi-well-based (24-well) electrochemical dissolved oxygen sensor (m-well ECDO sensor) array on a glass substrate, using Nafion as a solid polymer electrolyte. The designed m- well ECDO sensor array shares working electrodes, reference electrodes, and counter electrodes, to reduce the number of electrode pads to approximately one-fourth of its expected pad numbers (72 to 18), compared to the simply repeated 24 sensors. This m-well ECDO sensor array can simultaneously measure up to six sensors at individual crossing points of the row and column electrodes. The fabricated m-well ECDO sensor array showed good repeatability during five consecutive measurements at intervals of 100 s with a 2.4 mu m thick Nafion membrane. The response time was about 10 s when the solution state changed from the air-saturated state to the zero-oxygen state, and the measured output current was about -1 mu A and-20 nA in the corresponding states, respectively. The fabricated m-well ECDO sensor array was applied to measure the oxygen consumption rate of C2C12 cells for a cell metabolic study. The ratios of the uncoupled oxygen consumption rate (OCR) to the coupled OCR obtained from three sensors of the fabricated m-well ECDO sensor array were 2.16, 2.22, 2.18, which are higher than 1.71, 1.68, 1.73 obtained from of the XF-24 of Agilent Inc. in 5,000, 10,000, 30,000 cells, respectively. This indicates that using the fabricated m-well ECDO sensor array, variations in DO (dissolved oxygen) concentration due to cellular respiration can be effectively detected, even at low cell numbers. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE SA | - |
dc.subject | IN-VIVO | - |
dc.subject | MITOCHONDRIA | - |
dc.subject | NAFION(R) | - |
dc.subject | ELECTRODE | - |
dc.subject | MEMBRANE | - |
dc.subject | DEMAND | - |
dc.subject | DIFFERENTIATION | - |
dc.subject | FABRICATION | - |
dc.subject | REDUCTION | - |
dc.subject | THICKNESS | - |
dc.title | Development of multi-well-based electrochemical dissolved oxygen sensor array | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Pak, James Jungho | - |
dc.identifier.doi | 10.1016/j.snb.2019.127465 | - |
dc.identifier.scopusid | 2-s2.0-85076540419 | - |
dc.identifier.wosid | 000507459500010 | - |
dc.identifier.bibliographicCitation | SENSORS AND ACTUATORS B-CHEMICAL, v.306 | - |
dc.relation.isPartOf | SENSORS AND ACTUATORS B-CHEMICAL | - |
dc.citation.title | SENSORS AND ACTUATORS B-CHEMICAL | - |
dc.citation.volume | 306 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Electrochemistry | - |
dc.relation.journalResearchArea | Instruments & Instrumentation | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Electrochemistry | - |
dc.relation.journalWebOfScienceCategory | Instruments & Instrumentation | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | MITOCHONDRIA | - |
dc.subject.keywordPlus | NAFION(R) | - |
dc.subject.keywordPlus | ELECTRODE | - |
dc.subject.keywordPlus | MEMBRANE | - |
dc.subject.keywordPlus | DEMAND | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | FABRICATION | - |
dc.subject.keywordPlus | REDUCTION | - |
dc.subject.keywordPlus | THICKNESS | - |
dc.subject.keywordAuthor | Electrochemical sensor | - |
dc.subject.keywordAuthor | Dissolved oxygen sensor | - |
dc.subject.keywordAuthor | Multi-well based sensor array | - |
dc.subject.keywordAuthor | Oxygen consumption rate | - |
dc.subject.keywordAuthor | Cell metabolism | - |
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