Fusion tags to enhance heterologous protein expression
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ki, Mi-Ran | - |
dc.contributor.author | Pack, Seung Pil | - |
dc.date.accessioned | 2021-08-31T08:41:55Z | - |
dc.date.available | 2021-08-31T08:41:55Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2020-03 | - |
dc.identifier.issn | 0175-7598 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/57430 | - |
dc.description.abstract | Escherichia coli is the most widely used heterologous protein expression system. However, this system remains a challenge due to the low solubility of proteins, insufficient yield, and inclusion body formation. Numerous approaches have sought to address these issues. The use of a fusion tag is one of the most powerful strategies for obtaining large amounts of heterologous protein in E. coli expression system. Here, recent advances in fusion tags that increase the expression of proteins are reviewed. In addition, proposed concepts for designing peptide tags to increase protein expression are discussed. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | SPRINGER | - |
dc.subject | CELLULOSE-BINDING DOMAIN | - |
dc.subject | INCLUSION-BODY FORMATION | - |
dc.subject | HIGH-LEVEL EXPRESSION | - |
dc.subject | ESCHERICHIA-COLI | - |
dc.subject | RECOMBINANT PROTEINS | - |
dc.subject | ANTIMICROBIAL PEPTIDES | - |
dc.subject | TRANSLATION INITIATION | - |
dc.subject | CARBONIC-ANHYDRASE | - |
dc.subject | AFFINITY TAGS | - |
dc.subject | PURIFICATION | - |
dc.title | Fusion tags to enhance heterologous protein expression | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Ki, Mi-Ran | - |
dc.contributor.affiliatedAuthor | Pack, Seung Pil | - |
dc.identifier.doi | 10.1007/s00253-020-10402-8 | - |
dc.identifier.scopusid | 2-s2.0-85078485825 | - |
dc.identifier.wosid | 000517127300011 | - |
dc.identifier.bibliographicCitation | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, v.104, no.6, pp.2411 - 2425 | - |
dc.relation.isPartOf | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.citation.title | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.citation.volume | 104 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 2411 | - |
dc.citation.endPage | 2425 | - |
dc.type.rims | ART | - |
dc.type.docType | Review | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.subject.keywordPlus | CELLULOSE-BINDING DOMAIN | - |
dc.subject.keywordPlus | INCLUSION-BODY FORMATION | - |
dc.subject.keywordPlus | HIGH-LEVEL EXPRESSION | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | RECOMBINANT PROTEINS | - |
dc.subject.keywordPlus | ANTIMICROBIAL PEPTIDES | - |
dc.subject.keywordPlus | TRANSLATION INITIATION | - |
dc.subject.keywordPlus | CARBONIC-ANHYDRASE | - |
dc.subject.keywordPlus | AFFINITY TAGS | - |
dc.subject.keywordPlus | PURIFICATION | - |
dc.subject.keywordAuthor | Escherichia coli | - |
dc.subject.keywordAuthor | Heterologous protein expression | - |
dc.subject.keywordAuthor | Expression-enhancing tag | - |
dc.subject.keywordAuthor | Fusion tag | - |
dc.subject.keywordAuthor | Protein tag | - |
dc.subject.keywordAuthor | Peptide tag | - |
dc.subject.keywordAuthor | Solubility tag | - |
dc.subject.keywordAuthor | Aggregation-prone tag | - |
dc.subject.keywordAuthor | Inclusion body | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.