The immobilization of fibronectin- and fibroblast growth factor 2-derived peptides on a culture plate supports the attachment and proliferation of human pluripotent stem cells
DC Field | Value | Language |
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dc.contributor.author | Dayem, Ahmed Abdal | - |
dc.contributor.author | Won, Jihye | - |
dc.contributor.author | Goo, Hui-Gwan | - |
dc.contributor.author | Yang, Gwang-Mo | - |
dc.contributor.author | Seo, Dong Sik | - |
dc.contributor.author | Jeon, Byeong-Min | - |
dc.contributor.author | Choi, Hye Yeon | - |
dc.contributor.author | Park, Sang Eun | - |
dc.contributor.author | Lim, Kyung | - |
dc.contributor.author | Jang, Seon-Ho | - |
dc.contributor.author | Lee, Soo Bin | - |
dc.contributor.author | Choi, Sang Baek | - |
dc.contributor.author | Kim, Kyeongseok | - |
dc.contributor.author | Kan, Geun-Ho | - |
dc.contributor.author | Yeo, Gyu-Bum | - |
dc.contributor.author | Kim, Dae-Sung | - |
dc.contributor.author | Cho, Ssang-Goo | - |
dc.date.accessioned | 2021-08-31T08:44:40Z | - |
dc.date.available | 2021-08-31T08:44:40Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2020-03 | - |
dc.identifier.issn | 1873-5061 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/57451 | - |
dc.description.abstract | Pluripotent stem cells (PSCs) offer a promising tool for regenerative medicine. The clinical application of PSCs inevitably requires a large-scale culture in a highly defined environment. The present study aimed to devise defined coating materials for the efficient adhesion and proliferation of human PSCs (hPSCs). We tested the activity of seven fibronectin-derived peptides and three laminin-derived peptides for the attachment and proliferation of hPSCs through their immobilization on the bottom of culture dishes by creating a fusion protein with the mussel adhesion protein. Among the extracellular matrix (ECM) mimetics tested, one fibronectin-derived peptide, PHSRN-GRGDSP, significantly promoted adhesion, enhanced alkaline phosphatase activity, and increased pluripotency-related gene expression in hPSCs compared to Matrigel. Furthermore, co-immobilization of a particular canofin peptide derived from fibroblast growth factor 2 increased pluripotency marker expression, which may offer the possibility of culture without growth factor supplementation. Our findings afford a novel defined condition for the efficient culture of hPSCs and may be utilized in future clinical applications. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER | - |
dc.subject | SELF-RENEWAL | - |
dc.subject | ADHESION | - |
dc.subject | RGD | - |
dc.subject | VINCULIN | - |
dc.subject | ALPHA(5)BETA(1) | - |
dc.subject | ACTIVATION | - |
dc.subject | AGONISTS | - |
dc.subject | LAMININ | - |
dc.subject | GENES | - |
dc.subject | PHSRN | - |
dc.title | The immobilization of fibronectin- and fibroblast growth factor 2-derived peptides on a culture plate supports the attachment and proliferation of human pluripotent stem cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Dae-Sung | - |
dc.identifier.doi | 10.1016/j.scr.2020.101700 | - |
dc.identifier.scopusid | 2-s2.0-85078156686 | - |
dc.identifier.wosid | 000522734100014 | - |
dc.identifier.bibliographicCitation | STEM CELL RESEARCH, v.43 | - |
dc.relation.isPartOf | STEM CELL RESEARCH | - |
dc.citation.title | STEM CELL RESEARCH | - |
dc.citation.volume | 43 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Cell & Tissue Engineering | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.subject.keywordPlus | SELF-RENEWAL | - |
dc.subject.keywordPlus | ADHESION | - |
dc.subject.keywordPlus | RGD | - |
dc.subject.keywordPlus | VINCULIN | - |
dc.subject.keywordPlus | ALPHA(5)BETA(1) | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | AGONISTS | - |
dc.subject.keywordPlus | LAMININ | - |
dc.subject.keywordPlus | GENES | - |
dc.subject.keywordPlus | PHSRN | - |
dc.subject.keywordAuthor | Human induced pluripotent stem cell | - |
dc.subject.keywordAuthor | Stem cell | - |
dc.subject.keywordAuthor | Niche ECM motif | - |
dc.subject.keywordAuthor | Proliferation | - |
dc.subject.keywordAuthor | Pluripotency | - |
dc.subject.keywordAuthor | Adhesion | - |
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