Real-Time Measurement of the Liquid Amount in Cryo-Electron Microscopy Grids Using Laser Diffraction of Regular 2-D Holes of the Grids
DC Field | Value | Language |
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dc.contributor.author | Ahn, Jinsook | - |
dc.contributor.author | Lee, Dukwon | - |
dc.contributor.author | Jo, Inseong | - |
dc.contributor.author | Jeong, Hyeongseop | - |
dc.contributor.author | Hyun, Jae-Kyung | - |
dc.contributor.author | Woo, Jae-Sung | - |
dc.contributor.author | Choi, Sang-Ho | - |
dc.contributor.author | Ha, Nam-Chul | - |
dc.date.accessioned | 2021-08-31T08:49:18Z | - |
dc.date.available | 2021-08-31T08:49:18Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2020-03 | - |
dc.identifier.issn | 1016-8478 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/57485 | - |
dc.description.abstract | Cryo-electron microscopy (cryo-EM) is now the first choice to determine the high-resolution structures of huge protein complexes. Grids with two-dimensional arrays of holes covered with a carbon film are typically used in cryo-EM. Although semi-automatic plungers are available, notable trial-and-error is still required to obtain a suitable grid specimen. Herein, we introduce a new method to obtain thin ice specimens using real-time measurement of the liquid amounts in cryo-EM grids. The grids for cryo-EM strongly diffracted laser light, and the diffraction intensity of each spot was measurable in real-time. The measured diffraction patterns represented the states of the liquid in the holes due to the curvature of the liquid around them. Using the diffraction patterns, the optimal time point for freezing the grids for cryo-EM was obtained in real-time. This development will help researchers rapidly determine highresolution protein structures using the limited resource of cryo-EM instrument access. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | KOREAN SOC MOLECULAR & CELLULAR BIOLOGY | - |
dc.subject | CRYO-EM | - |
dc.subject | RESOLUTION | - |
dc.subject | SAMPLES | - |
dc.title | Real-Time Measurement of the Liquid Amount in Cryo-Electron Microscopy Grids Using Laser Diffraction of Regular 2-D Holes of the Grids | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Woo, Jae-Sung | - |
dc.identifier.doi | 10.14348/molcells.2020.2238 | - |
dc.identifier.scopusid | 2-s2.0-85082342594 | - |
dc.identifier.wosid | 000521825800010 | - |
dc.identifier.bibliographicCitation | MOLECULES AND CELLS, v.43, no.3, pp.298 - 303 | - |
dc.relation.isPartOf | MOLECULES AND CELLS | - |
dc.citation.title | MOLECULES AND CELLS | - |
dc.citation.volume | 43 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 298 | - |
dc.citation.endPage | 303 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART002569047 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.subject.keywordPlus | CRYO-EM | - |
dc.subject.keywordPlus | RESOLUTION | - |
dc.subject.keywordPlus | SAMPLES | - |
dc.subject.keywordAuthor | cryo electron microscopy | - |
dc.subject.keywordAuthor | grid preparation | - |
dc.subject.keywordAuthor | laser diffraction | - |
dc.subject.keywordAuthor | real-time measurement | - |
dc.subject.keywordAuthor | vitreous ice | - |
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