Calcium-doped mesoporous silica nanoparticles as a lysosomolytic nanocarrier for amine-free loading and cytosolic delivery of siRNA
DC Field | Value | Language |
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dc.contributor.author | Choi, Eunshil | - |
dc.contributor.author | Lim, Dong-Kwon | - |
dc.contributor.author | Kim, Sehoon | - |
dc.date.accessioned | 2021-08-31T12:05:58Z | - |
dc.date.available | 2021-08-31T12:05:58Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2020-01-25 | - |
dc.identifier.issn | 1226-086X | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/57956 | - |
dc.description.abstract | For efficacious gene therapeutics, cytosolic transport of the endocytosed siRNA is crucial, not to mention a non-toxic delivery carrier composition. In this paper, we report facile achievement of amine-free loading and lysosomolytic delivery of siRNA in an unconventional way by using calcium (Ca2+)-doped mesoporous silica nanoparticles (CMSNs) as a host material along with a pore-loaded endosomal disruptor, chloroquine (CQ). It is demonstrated that CMSNs are capable of direct siRNA loading through Ca2+-incorporated larger pores, as well as efficient release of the loaded siRNA under pH control thanks to the high degradability of the Ca2+-doped silica backbone. A therapeutic performance of siRNA-loaded CMSNs is exemplified in vitro with SKOV3 human ovarian cancer cells, which underwent distinct knockdown of a target anti-apoptotic Bcl-2 gene and consequent apoptosis after incubation with those particles. When co-loaded with CQ particles were shown to substantially promote the cytosolic delivery of the endocytosed siRNA via endo/lysosomal escape for more effective induction of cell apoptosis. The results suggest that a variety of target-genes can be applicable to the presented delivery system on demand, providing a highly versatile feature of our nanocarrier for many gene-therapeutic applications with higher efficacy. (C) 2019 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE INC | - |
dc.subject | CO-DELIVERY | - |
dc.subject | BCL-2 SIRNA | - |
dc.subject | RNA | - |
dc.subject | DOXORUBICIN | - |
dc.subject | INTERFERENCE | - |
dc.subject | RELEASE | - |
dc.subject | COLOCALIZATION | - |
dc.subject | NANOCAPSULES | - |
dc.subject | DEGRADATION | - |
dc.subject | SORAFENIB | - |
dc.title | Calcium-doped mesoporous silica nanoparticles as a lysosomolytic nanocarrier for amine-free loading and cytosolic delivery of siRNA | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lim, Dong-Kwon | - |
dc.contributor.affiliatedAuthor | Kim, Sehoon | - |
dc.identifier.doi | 10.1016/j.jiec.2019.08.054 | - |
dc.identifier.scopusid | 2-s2.0-85071763882 | - |
dc.identifier.wosid | 000501660000007 | - |
dc.identifier.bibliographicCitation | JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY, v.81, pp.71 - 80 | - |
dc.relation.isPartOf | JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY | - |
dc.citation.title | JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY | - |
dc.citation.volume | 81 | - |
dc.citation.startPage | 71 | - |
dc.citation.endPage | 80 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART002553214 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Engineering, Chemical | - |
dc.subject.keywordPlus | CO-DELIVERY | - |
dc.subject.keywordPlus | BCL-2 SIRNA | - |
dc.subject.keywordPlus | RNA | - |
dc.subject.keywordPlus | DOXORUBICIN | - |
dc.subject.keywordPlus | INTERFERENCE | - |
dc.subject.keywordPlus | RELEASE | - |
dc.subject.keywordPlus | COLOCALIZATION | - |
dc.subject.keywordPlus | NANOCAPSULES | - |
dc.subject.keywordPlus | DEGRADATION | - |
dc.subject.keywordPlus | SORAFENIB | - |
dc.subject.keywordAuthor | Mesoporous silica | - |
dc.subject.keywordAuthor | Calcium | - |
dc.subject.keywordAuthor | siRNA | - |
dc.subject.keywordAuthor | Gene silencing | - |
dc.subject.keywordAuthor | Endosomal escape | - |
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