Stabilized and Immobilized Carbonic Anhydrase on Electrospun Nanofibers for Enzymatic CO2 Conversion and Utilization in Expedited Microalgal Growth
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jun, Seung-Hyun | - |
dc.contributor.author | Yang, Jusang | - |
dc.contributor.author | Jeon, Hancheol | - |
dc.contributor.author | Kim, Han Sol | - |
dc.contributor.author | Pack, Seung Pil | - |
dc.contributor.author | Jin, EonSeon | - |
dc.contributor.author | Kim, Jungbae | - |
dc.date.accessioned | 2021-08-31T12:26:14Z | - |
dc.date.available | 2021-08-31T12:26:14Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2020-01-21 | - |
dc.identifier.issn | 0013-936X | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/57973 | - |
dc.description.abstract | Carbonic anhydrases convert CO2 to bicarbonate at a high turnover rate up to 10(6) s(-1), but their actual applications in CO2 conversion processes are hampered by their poor stability. This study reports highly loaded and stabilized bovine carbonic anhydrase (bCA) upon being immobilized onto electrospun polymer nanofibers in the form of enzyme precipitate coating (EPC). The EPC protocol, consisting of enzyme covalent attachment, precipitation, and cross-linking, maintained 65.3% of initial activity even after being incubated in aqueous solution at room temperature under shaking at 200 rpm for 868 days. EPC also showed strong resistance to the treatment of the metal chelation agent, ethylenediaminetetraacetic acid, and molecular dynamic simulation was carried out to elucidate the prevention of metal leaching from the active site of bCA upon being cross-linked in the form of EPC. Highly stable EPC with high bCA loading was employed for the conversion of bubbling CO2 to bicarbonate, and the bicarbonate solution was utilized as a carbon source for expedited microalgae growth in a separate bioreactor. The addition of EPC in the bubbling CO2 reactor resulted in 134 and 231% accelerated microalgae growths compared to the controls with and without 25 mM sodium bicarbonate, respectively. EPC with high enzyme loading and unprecedentedly successful stabilization of enzyme stability has a great potential to be used for the development of various enzyme-mediated CO2 conversion and utilization technologies. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.subject | DIOXIDE | - |
dc.subject | CAPTURE | - |
dc.subject | BIOMASS | - |
dc.subject | NANOBIOCATALYSIS | - |
dc.subject | OPPORTUNITIES | - |
dc.subject | MECHANISMS | - |
dc.subject | DIGESTION | - |
dc.subject | PRODUCTS | - |
dc.subject | BIOFUELS | - |
dc.subject | COATINGS | - |
dc.title | Stabilized and Immobilized Carbonic Anhydrase on Electrospun Nanofibers for Enzymatic CO2 Conversion and Utilization in Expedited Microalgal Growth | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Jungbae | - |
dc.identifier.doi | 10.1021/acs.est.9b05284 | - |
dc.identifier.scopusid | 2-s2.0-85078390612 | - |
dc.identifier.wosid | 000509419700061 | - |
dc.identifier.bibliographicCitation | ENVIRONMENTAL SCIENCE & TECHNOLOGY, v.54, no.2, pp.1223 - 1231 | - |
dc.relation.isPartOf | ENVIRONMENTAL SCIENCE & TECHNOLOGY | - |
dc.citation.title | ENVIRONMENTAL SCIENCE & TECHNOLOGY | - |
dc.citation.volume | 54 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 1223 | - |
dc.citation.endPage | 1231 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalResearchArea | Environmental Sciences & Ecology | - |
dc.relation.journalWebOfScienceCategory | Engineering, Environmental | - |
dc.relation.journalWebOfScienceCategory | Environmental Sciences | - |
dc.subject.keywordPlus | DIOXIDE | - |
dc.subject.keywordPlus | CAPTURE | - |
dc.subject.keywordPlus | BIOMASS | - |
dc.subject.keywordPlus | NANOBIOCATALYSIS | - |
dc.subject.keywordPlus | OPPORTUNITIES | - |
dc.subject.keywordPlus | MECHANISMS | - |
dc.subject.keywordPlus | DIGESTION | - |
dc.subject.keywordPlus | PRODUCTS | - |
dc.subject.keywordPlus | BIOFUELS | - |
dc.subject.keywordPlus | COATINGS | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.