mRNA and miRNA profiling of Zika virus-infected human umbilical cord mesenchymal stem cells identifies miR-142-5p as an antiviral factor
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Seong, Rak-Kyun | - |
dc.contributor.author | Lee, Jae Kyung | - |
dc.contributor.author | Cho, Geum Joon | - |
dc.contributor.author | Kumar, Mukesh | - |
dc.contributor.author | Shin, Ok Sarah | - |
dc.date.accessioned | 2021-08-31T14:47:45Z | - |
dc.date.available | 2021-08-31T14:47:45Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2020-01-01 | - |
dc.identifier.issn | 2222-1751 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/58387 | - |
dc.description.abstract | Zika virus (ZIKV) infection during pregnancy is associated with congenital brain abnormalities, a finding that highlights the urgent need to understand mother-to-fetus transmission mechanisms. Human umbilical cord mesenchymal stem cells (hUCMSCs) are susceptible to ZIKV infection but the underlying mechanisms of viral susceptibility remain largely unexplored. In this study, we have characterized and compared host mRNA and miRNA expression profiles in hUCMSCs after infection with two lineages of ZIKV, African (MR766) and Asian (PRVABC59). RNA sequencing analysis identified differentially expressed genes involved in anti-viral immunity and mitochondrial dynamics following ZIKV infection. In particular, ZIKV-infected hUCMSCs displayed mitochondrial elongation and the treatment of hUCMSCs with mitochondrial fission inhibitor led to a dose-dependent increase in ZIKV gene expression and decrease in anti-viral signalling pathways. Moreover, small RNA sequencing analysis identified several significantly up- or down-regulated microRNAs. Interestingly, miR-142-5p was significantly downregulated upon ZIKV infection, whereas cellular targets of miR-142-5p,IL6STandITGAV, were upregulated. Overexpression of miR-142-5p resulted in the suppression of ZIKV replication. Furthermore, blockingITGAVexpression resulted in a significant suppression of ZIKV binding to cells, suggesting a potential role of ITGAV in ZIKV entry. In conclusion, these results demonstrate both common and specific host responses to African and Asian ZIKV lineages and indicate miR-142-5p as a key regulator of ZIKV replication in the umbilical cords. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | TAYLOR & FRANCIS LTD | - |
dc.subject | INTEGRIN ALPHA(V)BETA(3) | - |
dc.subject | VIRULENT | - |
dc.title | mRNA and miRNA profiling of Zika virus-infected human umbilical cord mesenchymal stem cells identifies miR-142-5p as an antiviral factor | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Cho, Geum Joon | - |
dc.contributor.affiliatedAuthor | Shin, Ok Sarah | - |
dc.identifier.doi | 10.1080/22221751.2020.1821581 | - |
dc.identifier.scopusid | 2-s2.0-85091390399 | - |
dc.identifier.wosid | 000574429800001 | - |
dc.identifier.bibliographicCitation | EMERGING MICROBES & INFECTIONS, v.9, no.1, pp.2061 - 2075 | - |
dc.relation.isPartOf | EMERGING MICROBES & INFECTIONS | - |
dc.citation.title | EMERGING MICROBES & INFECTIONS | - |
dc.citation.volume | 9 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 2061 | - |
dc.citation.endPage | 2075 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Immunology | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Immunology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.subject.keywordPlus | INTEGRIN ALPHA(V)BETA(3) | - |
dc.subject.keywordPlus | VIRULENT | - |
dc.subject.keywordAuthor | ZIKV | - |
dc.subject.keywordAuthor | hUCMSCs | - |
dc.subject.keywordAuthor | miRNA | - |
dc.subject.keywordAuthor | innate immunity | - |
dc.subject.keywordAuthor | RNA-seq | - |
dc.subject.keywordAuthor | small RNA-seq | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.