Accelerated Osteogenic Differentiation of MC3T3-E1 Cells by Lactoferrin-Conjugated Nanodiamonds through Enhanced Anti-Oxidant and Anti-Inflammatory Effects
DC Field | Value | Language |
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dc.contributor.author | Kim, Sung Eun | - |
dc.contributor.author | Choi, Somang | - |
dc.contributor.author | Hong, Jae-Young | - |
dc.contributor.author | Shim, Kyu-Sik | - |
dc.contributor.author | Kim, Tae-Hoon | - |
dc.contributor.author | Park, Kyeongsoon | - |
dc.contributor.author | Lee, Suk-Ha | - |
dc.date.accessioned | 2021-08-31T15:04:24Z | - |
dc.date.available | 2021-08-31T15:04:24Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2020-01 | - |
dc.identifier.issn | 2079-4991 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/58498 | - |
dc.description.abstract | The purpose of this study was to investigate the effects of lactoferrin (LF)-conjugated nanodiamonds (NDs) in vitro on both anti-oxidant and anti-inflammation activity as well as osteogenic promotion. The application of LF-NDs resulted in sustained release of LF for up to 7 days. In vitro anti-oxidant analyses performed using Dichlorofluorescin diacetate (DCF-DA) assay and cell proliferation studies showed that LF (50 mu g)-NDs effectively scavenged the reactive oxygen species (ROS) in MC3T3-E1 cells (osteoblast-like cells) after H2O2 treatment and increased proliferation of cells after H2O2 treatment. Treatment of lipopolysaccharide (LPS)-induced MC3T3-E1 cells with LF-NDs suppressed levels of pro-inflammatory cytokines, including interleukin-1 beta (IL-1 beta) and tumor necrosis factor-alpha (TNF-alpha). In addition, LF-NDs were associated with outstanding enhancement of osteogenic activity of MC3T3-E1 cells due to increased alkaline phosphatase (ALP) and calcium deposition. Our findings suggest that LF-NDs are an important substrate for alleviating ROS effects and inflammation, as well as promoting osteogenic differentiation of cells. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.subject | OXIDATIVE STRESS | - |
dc.subject | BONE | - |
dc.subject | NANOPARTICLES | - |
dc.subject | MICROSPHERES | - |
dc.subject | COMPOSITES | - |
dc.subject | PROTEIN | - |
dc.subject | IMMUNE | - |
dc.title | Accelerated Osteogenic Differentiation of MC3T3-E1 Cells by Lactoferrin-Conjugated Nanodiamonds through Enhanced Anti-Oxidant and Anti-Inflammatory Effects | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Sung Eun | - |
dc.contributor.affiliatedAuthor | Hong, Jae-Young | - |
dc.identifier.doi | 10.3390/nano10010050 | - |
dc.identifier.scopusid | 2-s2.0-85077294144 | - |
dc.identifier.wosid | 000516825600050 | - |
dc.identifier.bibliographicCitation | NANOMATERIALS, v.10, no.1 | - |
dc.relation.isPartOf | NANOMATERIALS | - |
dc.citation.title | NANOMATERIALS | - |
dc.citation.volume | 10 | - |
dc.citation.number | 1 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalResearchArea | Physics | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Physics, Applied | - |
dc.subject.keywordPlus | OXIDATIVE STRESS | - |
dc.subject.keywordPlus | BONE | - |
dc.subject.keywordPlus | NANOPARTICLES | - |
dc.subject.keywordPlus | MICROSPHERES | - |
dc.subject.keywordPlus | COMPOSITES | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | IMMUNE | - |
dc.subject.keywordAuthor | nanodiamonds | - |
dc.subject.keywordAuthor | lactoferrin | - |
dc.subject.keywordAuthor | anti-oxidant | - |
dc.subject.keywordAuthor | anti-inflammatory | - |
dc.subject.keywordAuthor | osteogenic differentiation | - |
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