Detailed Information

Cited 0 time in webofscience Cited 0 time in scopus
Metadata Downloads

Specificity Assessment of CRISPR Genome Editing of Oncogenic EGFR Point Mutation with Single-Base Differences

Authors
Bae, TaegeunKim, HanseopKim, Jeong HeeKim, Yong JunLee, Seung HwanHam, Byung-JooHur, Junho K.
Issue Date
1월-2020
Publisher
MDPI
Keywords
CRISPR; off-target; specificity; PAM; single-base precision
Citation
MOLECULES, v.25, no.1
Indexed
SCIE
SCOPUS
Journal Title
MOLECULES
Volume
25
Number
1
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/58541
DOI
10.3390/molecules25010052
ISSN
1420-3049
Abstract
In CRISPR genome editing, CRISPR proteins form ribonucleoprotein complexes with guide RNAs to bind and cleave the target DNAs with complete sequence complementarity. CRISPR genome editing has a high potential for use in precision gene therapy for various diseases, including cancer and genetic disorders, which are caused by DNA mutations within the genome. However, several studies have shown that targeting the DNA via sequence complementarity is imperfect and subject to unintended genome editing of other genomic loci with similar sequences. These off-target problems pose critical safety issues in the therapeutic applications of CRISPR technology, with particular concerns in terms of the genome editing of pathogenic point mutations, where non-mutant alleles can become an off-target with only a one-base difference. In this study, we sought to assess a novel CRISPR genome editing technique that has been proposed to achieve a high specificity by positioning the mismatches within the protospacer adjacent motif (PAM) sequence. To this end, we compared the genome editing specificities of the PAM-based and conventional methods on an oncogenic single-base mutation in the endothelial growth factor receptor (EGFR). The results indicated that the PAM-based method provided a significantly increased genome editing specificity for pathogenic mutant alleles with single-base precision.
Files in This Item
There are no files associated with this item.
Appears in
Collections
Graduate School > Department of Biomedical Sciences > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher Ham, Byung Joo photo

Ham, Byung Joo
의과학과
Read more

Altmetrics

Total Views & Downloads

BROWSE