Development of a neutralization assay based on the pseudotyped chikungunya virus of a Korean isolate
DC Field | Value | Language |
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dc.contributor.author | Chung, Woo-Chang | - |
dc.contributor.author | Hwang, Kwang Yeon | - |
dc.contributor.author | Kang, Suk-Jo | - |
dc.contributor.author | Kim, Jae-Ouk | - |
dc.contributor.author | Song, Moon Jung | - |
dc.date.accessioned | 2021-08-31T15:14:58Z | - |
dc.date.available | 2021-08-31T15:14:58Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2020-01 | - |
dc.identifier.issn | 1225-8873 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/58567 | - |
dc.description.abstract | The Chikungunya virus (CHIKV) belongs to the Alphavirus genus of Togaviridae family and contains a positive-sense single stranded RNA genome. Infection by this virus mainly causes sudden high fever, rashes, headache, and severe joint pain that can last for several months or years. CHIKV, a mosquito-borne arbovirus, is considered a re-emerging pathogen that has become one of the most pressing global health concerns due to a rapid increase in epidemics. Because handling of CHIKV is restricted to Biosafety Level 3 (BSL-3) facilities, the evaluation of prophylactic vaccines or antivirals has been substantially hampered. In this study, we first iden-tified the whole structural polyprotein sequence of a CHIKV strain isolated in South Korea (KNIH/2009/77). Phylogenetic analysis showed that this sequence clustered within the East/ Central/South African CHIKV genotype. Using this sequence information, we constructed a CHIKV-pseudotyped lenti-virus expressing the structural polyprotein of the Korean CHIKV isolate (CHIKVpseudo) and dual reporter genes of green fluorescence protein and luciferase. We then developed a pseudovirus-based neutralization assay (PBNA) using CHIKVpseudo. Results from this assay compared to those from the conventional plaque reduction neutralization test showed that our PBNA was a reliable and rapid method to evaluate the efficacy of neutralizing antibodies. More importantly, the neutralizing activities of human sera from CHIKV-infected individuals were quantitated by PBNA using CHIKVpseudo. Taken together, these results suggest that our PBNA for CHIKV may serve as a useful and safe method for testing the neutralizing activity of antibodies against CHIKV in BSL-2 facilities. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | MICROBIOLOGICAL SOCIETY KOREA | - |
dc.subject | REEMERGENCE | - |
dc.subject | MATURATION | - |
dc.subject | INFECTION | - |
dc.subject | VECTOR | - |
dc.title | Development of a neutralization assay based on the pseudotyped chikungunya virus of a Korean isolate | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Song, Moon Jung | - |
dc.identifier.doi | 10.1007/s12275-020-9384-0 | - |
dc.identifier.scopusid | 2-s2.0-85076107087 | - |
dc.identifier.wosid | 000511694400007 | - |
dc.identifier.bibliographicCitation | JOURNAL OF MICROBIOLOGY, v.58, no.1, pp.46 - 53 | - |
dc.relation.isPartOf | JOURNAL OF MICROBIOLOGY | - |
dc.citation.title | JOURNAL OF MICROBIOLOGY | - |
dc.citation.volume | 58 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 46 | - |
dc.citation.endPage | 53 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART002537618 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.subject.keywordPlus | REEMERGENCE | - |
dc.subject.keywordPlus | MATURATION | - |
dc.subject.keywordPlus | INFECTION | - |
dc.subject.keywordPlus | VECTOR | - |
dc.subject.keywordAuthor | Chikungunya virus | - |
dc.subject.keywordAuthor | Korean isolate | - |
dc.subject.keywordAuthor | Pseudovirus | - |
dc.subject.keywordAuthor | Neutralization assay | - |
dc.subject.keywordAuthor | Human serum | - |
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