PEGylated Bilirubin-coated Iron Oxide Nanoparticles as a Biosensor for Magnetic Relaxation Switching-based ROS Detection in Whole Blood
DC Field | Value | Language |
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dc.contributor.author | Lee, Dong Yun | - |
dc.contributor.author | Kang, Sukmo | - |
dc.contributor.author | Lee, Yonghyun | - |
dc.contributor.author | Kim, Jin Yong | - |
dc.contributor.author | Yoo, Dohyun | - |
dc.contributor.author | Jung, Wonsik | - |
dc.contributor.author | Lee, Soyoung | - |
dc.contributor.author | Jeong, Yong Yeon | - |
dc.contributor.author | Lee, Kwangyeol | - |
dc.contributor.author | Jon, Sangyong | - |
dc.date.accessioned | 2021-08-31T16:15:22Z | - |
dc.date.available | 2021-08-31T16:15:22Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2020 | - |
dc.identifier.issn | 1838-7640 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/59069 | - |
dc.description.abstract | Rationale: Magnetic relaxation switching (MRSw) induced by target-triggered aggregation or dissociation of superparamagnetic iron oxide nanoparticles (SPIONs) have been utilized for detection of diverse biomarkers. However, an MRSw-based biosensor for reactive oxygen species (ROS) has never been documented. Methods: To this end, we constructed a biosensor for ROS detection based on PEGylated bilirubin (PEG-BR)-coated SPIONs (PEG-BR@SPIONs) that were prepared by simple sonication via ligand exchange. In addition, near infra-red (NIR) fluorescent dye was loaded onto PEG-BR@SPIONs as a secondary option for fluorescence-based ROS detection. Results: PEG-BR@SPIONs showed high colloidal stability under physiological conditions, but upon exposure to the model ROS, NaOCl, in vitro, they aggregated, causing a decrease in signal intensity in T2-weighted MR images. Furthermore, ROS-responsive PEG-BR@SPIONs were taken up by lipopolysaccharide (LPS)-activated macrophages to a much greater extent than ROS-unresponsive control nanoparticles (PEG-DSPE@SPIONs). In a sepsis-mimetic clinical setting, PEG-BR@SPIONs were able to directly detect the concentrations of ROS in whole blood samples through a clear change in T2 MR signals and a 'turn-on' signal of fluorescence. Conclusions: These findings suggest that PEG-BR@SPIONs have the potential as a new type of dual mode (MRSw-based and fluorescence-based) biosensors for ROS detection and could be used to diagnose many diseases associated with ROS overproduction. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | IVYSPRING INT PUBL | - |
dc.subject | REACTIVE OXYGEN | - |
dc.subject | PHOTOTHERMAL THERAPY | - |
dc.subject | HUMAN-NEUTROPHILS | - |
dc.subject | CHLORINATION | - |
dc.subject | PHAGOCYTOSIS | - |
dc.subject | INFLAMMATION | - |
dc.title | PEGylated Bilirubin-coated Iron Oxide Nanoparticles as a Biosensor for Magnetic Relaxation Switching-based ROS Detection in Whole Blood | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Kwangyeol | - |
dc.identifier.doi | 10.7150/thno.39662 | - |
dc.identifier.scopusid | 2-s2.0-85078221743 | - |
dc.identifier.wosid | 000508008300002 | - |
dc.identifier.bibliographicCitation | THERANOSTICS, v.10, no.5, pp.1997 - 2007 | - |
dc.relation.isPartOf | THERANOSTICS | - |
dc.citation.title | THERANOSTICS | - |
dc.citation.volume | 10 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 1997 | - |
dc.citation.endPage | 2007 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.subject.keywordPlus | REACTIVE OXYGEN | - |
dc.subject.keywordPlus | PHOTOTHERMAL THERAPY | - |
dc.subject.keywordPlus | HUMAN-NEUTROPHILS | - |
dc.subject.keywordPlus | CHLORINATION | - |
dc.subject.keywordPlus | PHAGOCYTOSIS | - |
dc.subject.keywordPlus | INFLAMMATION | - |
dc.subject.keywordAuthor | Bilirubin nanoparticles | - |
dc.subject.keywordAuthor | Biosensors | - |
dc.subject.keywordAuthor | Iron oxide nanoparticles | - |
dc.subject.keywordAuthor | Magnetic relaxation switching | - |
dc.subject.keywordAuthor | Reactive oxygen species | - |
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