Sandaracinobacter neustonicus sp. nov., isolated from the sea surface microlayer in the Southwestern Pacific Ocean, and emended description of the genus Sandaracinobacter

Abstract

A Gram stain negative, non-motile, facultatively anaerobic and rod-shaped bacterial strain, designated PAMC 28131(T), was isolated from a sea surface microlayer sample in the open water of the Pacific Ocean. Phylogenetic analysis of the 16S rRNA gene sequence of strain PAMC 28131(T) revealed an affiliation to the genus Sandaracinobacter with the closest species Sandaracinobacter sibiricus RB16-17(T) (sequence similarity of 98.2%). Strain PAMC 28131(T) was able to grow optimally with 0.5-1.0% NaCl and at pH 6.5-7.0 and 30 degrees C. The polar lipids were phosphatidylglycerol, phosphatidylethanolamine, two unidentified phospholipids, an unidentified aminolipid, an unidentified glycolipid and an unidentified lipid. The major cellular fatty acids (>10%) were C-18:1 omega 6c and/or C-18:1 omega 7c, (42.6%), C-17:1 omega 6c (19.3%) and C-16:1 omega 6c and/or C-16:1 omega 7c (15.8%), and the respiratory quinone was Q-10. The genomic DNA G+C content was 65.3mol%. The phylogenetic, phenotypic and chemotaxonomic data showed that strain PAMC 28131(T) could be clearly distinguished from S. sibiricus RB16-17(T). Thus, strain PAMC 28131(T) should be classified as representing a novel species in the genus Sandaracinobacter, for which the name Sandaracinobacter neustonicus sp. nov. is proposed. The type strain is PAMC 28131(T) (=KCCM 43127(T) =JCM 30734(T)).