PVDF Nanofiber Scaffold Coated with a Vitronectin Peptide Facilitates the Neural Differentiation of Human Embryonic Stem CellsPVDF Nanofiber Scaffold Coated with a Vitronectin Peptide Facilitates the Neural Differentiation of Human Embryonic Stem Cells
- Other Titles
- PVDF Nanofiber Scaffold Coated with a Vitronectin Peptide Facilitates the Neural Differentiation of Human Embryonic Stem Cells
- Authors
- Byeong-Min Jeon; Gyu-Bum Yeon; Hui-Gwan Goo; Kyung Eun Lee; 김대성
- Issue Date
- 2020
- Publisher
- 한국발생생물학회
- Keywords
- Polyvinylidene fluoride (PVDF); Nanofiber; Vitronectin; Neural differentiation; hESCs; Dual-SMAD inhibition
- Citation
- 발생과 생식, v.24, no.2, pp.135 - 147
- Indexed
- KCI
- Journal Title
- 발생과 생식
- Volume
- 24
- Number
- 2
- Start Page
- 135
- End Page
- 147
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/59708
- ISSN
- 2465-9525
- Abstract
- Polyvinylidene fluoride (PVDF) is a stable and biocompatible material that has been broadly used in biomedical applications. Due to its piezoelectric property, the electrospun nanofiber of PVDF has been used to culture electroactive cells, such as osteocytes and cardiomyocytes.
Here, taking advantage of the piezoelectric property of PVDF, we have fabricated a PVDF nanofiber scaffolds using an electrospinning technique for differentiating human embryonic stem cells (hESCs) into neural precursors (NPs). Surface coating with a peptide derived from vitronectin enables hESCs to firmly adhere onto the nanofiber scaffolds and differentiate into NPs under dual-SMAD inhibition. Our nanofiber scaffolds supported the differentiation of hESCs into SOX1-positive NPs more significantly than Matrigel. The NPs generated on the nanofiber scaffolds could give rise to neurons, astrocytes, and oligodendrocyte precursors.
Furthermore, comparative transcriptome analysis revealed the variable expressions of 27 genes in the nanofiber scaffold groups, several of which are highly related to the biological processes required for neural differentiation. These results suggest that a PVDF nanofiber scaffold coated with a vitronectin peptide can serve as a highly efficient and defined culture platform for the neural differentiation of hESCs.
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