Enzymatic synthesis of l-fucose from L-fuculose using a fucose isomerase from Raoultella sp. and the biochemical and structural analyses of the enzyme
DC Field | Value | Language |
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dc.contributor.author | Kim, In Jung | - |
dc.contributor.author | Kim, Do Hyoung | - |
dc.contributor.author | Nam, Ki Hyun | - |
dc.contributor.author | Kim, Kyoung Heon | - |
dc.date.accessioned | 2021-08-31T21:25:02Z | - |
dc.date.available | 2021-08-31T21:25:02Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2019-12-05 | - |
dc.identifier.issn | 1754-6834 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/60950 | - |
dc.description.abstract | Background: L-Fucose is a rare sugar with potential uses in the pharmaceutical, cosmetic, and food industries. The enzymatic approach using L-fucose isomerase, which interconverts L-fucose and L-fuculose, can be an efficient way of producing L-fucose for industrial applications. Here, we performed biochemical and structural analyses of L-fucose isomerase identified from a novel species of Raoultella (RdFucI). Results: RdFucI exhibited higher enzymatic activity for L-fuculose than for L-fucose, and the rate for the reverse reaction of converting L-fuculose to L-fucose was higher than that for the forward reaction of converting L-fucose to L-fuculose. In the equilibrium mixture, a much higher proportion of L-fucose (similar to ninefold) was achieved at 30 degrees C and pH 7, indicating that the enzyme-catalyzed reaction favors the formation of L-fucose from L-fuculose. When biochemical analysis was conducted using L-fuculose as the substrate, the optimal conditions for RdFucI activity were determined to be 40 degrees C and pH 10. However, the equilibrium composition was not affected by reaction temperature in the range of 30 to 50 degrees C. Furthermore, RdFucI was found to be a metalloenzyme requiring Mn2+ as a cofactor. The comparative crystal structural analysis of RdFucI revealed the distinct conformation of alpha 7-alpha 8 loop of RdFucI. The loop is present at the entry of the substrate binding pocket and may affect the catalytic activity. Conclusions: RdFucI-catalyzed isomerization favored the reaction from L-fuculose to L-fucose. The biochemical and structural data of RdFucI will be helpful for the better understanding of the molecular mechanism of L-FucIs and the industrial production of L-fucose. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | BMC | - |
dc.subject | CONTAINING SULFATED POLYSACCHARIDES | - |
dc.subject | D-ARABINOSE ISOMERASE | - |
dc.subject | ISOMERIZES L-FUCOSE | - |
dc.subject | SUBSTRATE-SPECIFICITY | - |
dc.subject | D-ALTROSE | - |
dc.subject | OLIGOSACCHARIDES | - |
dc.subject | PROLIFERATION | - |
dc.subject | HYDROLYSIS | - |
dc.subject | KLEBSIELLA | - |
dc.subject | FUCOIDAN | - |
dc.title | Enzymatic synthesis of l-fucose from L-fuculose using a fucose isomerase from Raoultella sp. and the biochemical and structural analyses of the enzyme | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Kyoung Heon | - |
dc.identifier.doi | 10.1186/s13068-019-1619-0 | - |
dc.identifier.scopusid | 2-s2.0-85076491031 | - |
dc.identifier.wosid | 000510436600003 | - |
dc.identifier.bibliographicCitation | BIOTECHNOLOGY FOR BIOFUELS, v.12, no.1 | - |
dc.relation.isPartOf | BIOTECHNOLOGY FOR BIOFUELS | - |
dc.citation.title | BIOTECHNOLOGY FOR BIOFUELS | - |
dc.citation.volume | 12 | - |
dc.citation.number | 1 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Energy & Fuels | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Energy & Fuels | - |
dc.subject.keywordPlus | CONTAINING SULFATED POLYSACCHARIDES | - |
dc.subject.keywordPlus | D-ARABINOSE ISOMERASE | - |
dc.subject.keywordPlus | ISOMERIZES L-FUCOSE | - |
dc.subject.keywordPlus | SUBSTRATE-SPECIFICITY | - |
dc.subject.keywordPlus | D-ALTROSE | - |
dc.subject.keywordPlus | OLIGOSACCHARIDES | - |
dc.subject.keywordPlus | PROLIFERATION | - |
dc.subject.keywordPlus | HYDROLYSIS | - |
dc.subject.keywordPlus | KLEBSIELLA | - |
dc.subject.keywordPlus | FUCOIDAN | - |
dc.subject.keywordAuthor | L-Fucose | - |
dc.subject.keywordAuthor | L-Fuculose | - |
dc.subject.keywordAuthor | L-Fucose isomerase | - |
dc.subject.keywordAuthor | Raoultella | - |
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