Detailed Information

Cited 0 time in webofscience Cited 0 time in scopus
Metadata Downloads

Transgelin Depletion is Critical for the TGF beta 1-mediated Initiation of PLC gamma 1-Cofilin-driven Morphological and Migratory Changes in MDA-MB-231 Cells

Authors
Raymundo, Bernardo R.Oh, In-RokKim, MiJungKim, Chan-Wha
Issue Date
12월-2019
Publisher
WILEY-V C H VERLAG GMBH
Keywords
Actin cytoskeleton; Cofilin; Plasticity
Citation
BULLETIN OF THE KOREAN CHEMICAL SOCIETY, v.40, no.12, pp.1191 - 1201
Indexed
SCIE
SCOPUS
KCI
Journal Title
BULLETIN OF THE KOREAN CHEMICAL SOCIETY
Volume
40
Number
12
Start Page
1191
End Page
1201
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/61349
DOI
10.1002/bkcs.11900
ISSN
0253-2964
Abstract
The role of transgelin (TAGLN) in cancer has been discussed; however, the mechanisms underlying its regulation and correlation with MDA-MB-231 cell plasticity and migratory patterns remain unclear. We generated stable TAGLN-knockdown MDA-MB-231 cells and treated them with phorbol 12-myristate 13-acetate or transforming growth factor (TGF)-beta. Chemotaxis, morphology, and invasion were assayed using three-dimensional matrices to evaluate cytoskeletal remodeling and migratory changes. Wound healing assays were conducted using cell inserts. TAGLN knockdown cells exhibited altered morphology due to cytoskeletal remodeling, yet only untreated and TGF beta 1-treated cells exhibited enhanced migration. Untreated and TGF beta 1-treated TAGLN knockdown cells showed increased N-WASP, ROCK1, and ROCK2 protein levels, which induce cytoskeletal remodeling. Evaluating phospholipase C gamma 1 (PLC gamma 1)-cofilin signaling-related proteins revealed that only TGF beta 1-treated TAGLN knockdown cells were influenced by PLC gamma 1-cofilin signaling. Taken together, TAGLN knockdown is necessary for the TGF beta 1-mediated activation of PLC gamma 1-cofilin pathway-driven amoeboid morphology and enhanced migratory properties in MDA-MB-231 cells.
Files in This Item
There are no files associated with this item.
Appears in
Collections
College of Life Sciences and Biotechnology > Division of Life Sciences > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Altmetrics

Total Views & Downloads

BROWSE