Detailed Information

Cited 0 time in webofscience Cited 0 time in scopus
Metadata Downloads

Low-Cost Cultivation and Sporulation of Alkaliphilic Bacillus sp. Strain AK13 for Self-Healing Concrete

Full metadata record
DC Field Value Language
dc.contributor.authorHong, Minyoung-
dc.contributor.authorKim, Wonjae-
dc.contributor.authorPark, Woojun-
dc.date.accessioned2021-08-31T22:44:20Z-
dc.date.available2021-08-31T22:44:20Z-
dc.date.created2021-06-19-
dc.date.issued2019-12-
dc.identifier.issn1017-7825-
dc.identifier.urihttps://scholar.korea.ac.kr/handle/2021.sw.korea/61393-
dc.description.abstractThe alkaliphilic, calcium carbonate precipitating Bacillus sp. strain AK13 can be utilized in concrete for self-repairing. A statistical experimental design was used to develop an economical medium for its mass cultivation and sporulation. Two types of screening experiment were first conducted to identify substrates that promote the growth of the AK13 strain:the first followed a one-factor-at-a-time factorial design and the second a two-level full factorial design. Based on these screening experiments, barley malt powder and mixed grain powder were identified as the substrates that most effectively promoted the growth of the AK13 strain from a range of 21 agricultural products and by-products. A quadratic statistical model was then constructed using a central composite design and the concentration of the two substrates was optimized. The estimated growth and sporulation of Bacillus sp. strain AK13 in the proposed medium were 3.08 +/- 0.38 x 10(8) and 1.25 +/- 0.12 x 10(8) CFU/ml, respectively, which meant that the proposed low-cost medium was approximately 45 times more effective than the commercial medium in terms of the number of cultivatable bacteria per unit price. The spores were then powdered via a spray-drying process to produce a spore powder with a spore count of 2.0 +/- 0.7 x 10(9) CFU/g. The AK13 spore powder was mixed with cement paste, yeast extract, calcium lactate, and water. The yeast extract and calcium lactate generated the highest CFU/ml for AK13 at a 0.4:0.4 ratio compared to 0.4:0.25 (the original ratio of the B4 medium) and 0.4:0.8. Twenty-eight days after the spores were mixed into the mortar, the number of vegetative cells and spores of the AK13 strain had reached 10(6) CFU/g within the mortar. Cracks in the mortar under 0.29 mm were healed in 14 days. Calcium carbonate precipitation was observed on the crack surface. The mortar containing the spore powder was thus concluded to be effective in terms of healing micro-cracks.-
dc.languageEnglish-
dc.language.isoen-
dc.publisherKOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY-
dc.subjectSPORE PRODUCTION-
dc.subjectCULTURE-CONDITIONS-
dc.subjectBIOMASS PRODUCTION-
dc.subjectBACTERIA-
dc.subjectOPTIMIZATION-
dc.subjectSUBTILIS-
dc.subjectPASTEURII-
dc.subjectDESIGNS-
dc.subjectMEDIA-
dc.subjectACID-
dc.titleLow-Cost Cultivation and Sporulation of Alkaliphilic Bacillus sp. Strain AK13 for Self-Healing Concrete-
dc.typeArticle-
dc.contributor.affiliatedAuthorPark, Woojun-
dc.identifier.doi10.4014/jmb.1908.08034-
dc.identifier.scopusid2-s2.0-85077348460-
dc.identifier.wosid000504754500014-
dc.identifier.bibliographicCitationJOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.29, no.12, pp.1982 - 1992-
dc.relation.isPartOfJOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY-
dc.citation.titleJOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY-
dc.citation.volume29-
dc.citation.number12-
dc.citation.startPage1982-
dc.citation.endPage1992-
dc.type.rimsART-
dc.type.docTypeArticle-
dc.identifier.kciidART002535418-
dc.description.journalClass1-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.description.journalRegisteredClasskci-
dc.relation.journalResearchAreaBiotechnology & Applied Microbiology-
dc.relation.journalResearchAreaMicrobiology-
dc.relation.journalWebOfScienceCategoryBiotechnology & Applied Microbiology-
dc.relation.journalWebOfScienceCategoryMicrobiology-
dc.subject.keywordPlusSPORE PRODUCTION-
dc.subject.keywordPlusCULTURE-CONDITIONS-
dc.subject.keywordPlusBIOMASS PRODUCTION-
dc.subject.keywordPlusBACTERIA-
dc.subject.keywordPlusOPTIMIZATION-
dc.subject.keywordPlusSUBTILIS-
dc.subject.keywordPlusPASTEURII-
dc.subject.keywordPlusDESIGNS-
dc.subject.keywordPlusMEDIA-
dc.subject.keywordPlusACID-
dc.subject.keywordAuthorEconomical medium-
dc.subject.keywordAuthoragricultural products-
dc.subject.keywordAuthorstatistical design of experiments-
dc.subject.keywordAuthorspray dryer-
dc.subject.keywordAuthorcrack healing material-
dc.subject.keywordAuthorcalcium carbonate precipitation-
Files in This Item
There are no files associated with this item.
Appears in
Collections
College of Life Sciences and Biotechnology > Division of Environmental Science and Ecological Engineering > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher Park, Woo jun photo

Park, Woo jun
College of Life Sciences and Biotechnology (Division of Environmental Science and Ecological Engineering)
Read more

Altmetrics

Total Views & Downloads

BROWSE