Synergistic Autophagy Effect of miR-212-3p in Zoledronic Acid-Treated In Vitro and Orthotopic In Vivo Models and in Patient-Derived Osteosarcoma Cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Oh, Ju Yeon | - |
dc.contributor.author | Kim, Eun Ho | - |
dc.contributor.author | Lee, Yeon-Joo | - |
dc.contributor.author | Sai, Sei | - |
dc.contributor.author | Lim, Sun Ha | - |
dc.contributor.author | Park, Jang Woo | - |
dc.contributor.author | Chung, Hye Kyung | - |
dc.contributor.author | Kim, Joon | - |
dc.contributor.author | Vares, Guillaume | - |
dc.contributor.author | Takahashi, Akihisa | - |
dc.contributor.author | Jeong, Youn Kyoung | - |
dc.contributor.author | Kim, Mi-Sook | - |
dc.contributor.author | Kong, Chang-Bae | - |
dc.date.accessioned | 2021-09-01T01:14:07Z | - |
dc.date.available | 2021-09-01T01:14:07Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2019-11 | - |
dc.identifier.issn | 2072-6694 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/61999 | - |
dc.description.abstract | Osteosarcoma (OS) originates from osteoid bone tissues and is prone to metastasis, resulting in a high mortality rate. Although several treatments are available for OS, an effective cure does not exist for most patients with advanced OS. Zoledronic acid (ZOL) is a third-generation bisphosphonate that inhibits osteoclast-mediated bone resorption and has shown efficacy in treating bone metastases in patients with various types of solid tumors. Here, we sought to clarify the mechanisms through which ZOL inhibits OS cell proliferation. ZOL treatment inhibited OS cell proliferation, viability, and colony formation. Autophagy inhibition by RNA interference against Beclin-1 or ATG5 inhibited ZOL-induced OS cell death. ZOL induced autophagy by repressing the protein kinase B/mammalian target of rapamycin/p70S6 kinase pathway and extracellular signal-regulated kinase signaling-dependent autophagy in OS cell lines and patient-derived OS cells. Microarrays of miRNA showed that ZOL increased the levels of miR-212-3p, which is known to play an important role in autophagy, in OS in vitro and in vivo systems. Collectively, our data provided mechanistic insight into how increased miR-212-3p through ZOL treatment induces autophagy synergistically in OS cells, providing a preclinical rationale for conducting a broad-scale clinical evaluation of ZOL + miR-212-3p in treating OS. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.subject | BREAST-CANCER | - |
dc.subject | SKELETAL COMPLICATIONS | - |
dc.subject | MULTIPLE-MYELOMA | - |
dc.subject | BONE METASTASES | - |
dc.subject | DOUBLE-BLIND | - |
dc.subject | APOPTOSIS | - |
dc.subject | CARCINOMA | - |
dc.subject | GROWTH | - |
dc.subject | PHOSPHORYLATION | - |
dc.subject | PAMIDRONATE | - |
dc.title | Synergistic Autophagy Effect of miR-212-3p in Zoledronic Acid-Treated In Vitro and Orthotopic In Vivo Models and in Patient-Derived Osteosarcoma Cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Joon | - |
dc.identifier.doi | 10.3390/cancers11111812 | - |
dc.identifier.scopusid | 2-s2.0-85075192007 | - |
dc.identifier.wosid | 000502290100194 | - |
dc.identifier.bibliographicCitation | CANCERS, v.11, no.11 | - |
dc.relation.isPartOf | CANCERS | - |
dc.citation.title | CANCERS | - |
dc.citation.volume | 11 | - |
dc.citation.number | 11 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Oncology | - |
dc.relation.journalWebOfScienceCategory | Oncology | - |
dc.subject.keywordPlus | BREAST-CANCER | - |
dc.subject.keywordPlus | SKELETAL COMPLICATIONS | - |
dc.subject.keywordPlus | MULTIPLE-MYELOMA | - |
dc.subject.keywordPlus | BONE METASTASES | - |
dc.subject.keywordPlus | DOUBLE-BLIND | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.subject.keywordPlus | CARCINOMA | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordPlus | PHOSPHORYLATION | - |
dc.subject.keywordPlus | PAMIDRONATE | - |
dc.subject.keywordAuthor | zoledronic acid | - |
dc.subject.keywordAuthor | osteosarcoma | - |
dc.subject.keywordAuthor | autophagy | - |
dc.subject.keywordAuthor | miR-212 | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.