Role of Matrix Metalloproteinases 7 in the Pathogenesis of Laryngopharyngeal Reflux: Decreased E-cadherin in Acid exposed Primary Human Pharyngeal Epithelial Cells
- Authors
- Im, Nu-Ri; Lee, Doh Young; Kim, Byoungjae; Kim, Jian; Jung, Kwang-Yoon; Kim, Tae Hoon; Baek, Seung-Kuk
- Issue Date
- 11월-2019
- Publisher
- MDPI
- Keywords
- E-cadherin; matrix metalloproteinase; laryngopharyngeal reflux; acid; permeability
- Citation
- INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v.20, no.21
- Indexed
- SCIE
SCOPUS
- Journal Title
- INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
- Volume
- 20
- Number
- 21
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/62039
- DOI
- 10.3390/ijms20215276
- ISSN
- 1661-6596
- Abstract
- Cleavage of E-cadherin and the resultant weakness in the cell-cell links in the laryngeal epithelium lining is induced by exposure to acidic contents of the refluxate. Herein, we aimed to evaluate the role of matrix metalloproteinases (MMPs) in inducing E-cadherin level changes following acid exposure to the human pharyngeal mucosal cells. E-cadherin levels were inversely correlated with the duration of acid exposure. Treatment with actinonin, a broad MMP inhibitor, inhibited this change. Immunocytochemical staining and transepithelial permeability test revealed that the cell surface staining of E-cadherin decreased and transepithelial permeability increased after acid exposure, which was significantly inhibited by the MMP inhibitor. Among the various MMPs analyzed, the mRNA for MMP-7 in the cellular component was upregulated, and the secretion and enzymatic activity of MMP-7 in the culture media increased with the acid treatment. Consequently, MMP-7 plays a significant role in the degradation of E-cadherin after exposure to a relatively weak acidic condition that would be similar to the physiologic condition that occurs in Laryngopharyngeal reflux disease patients.
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Collections - College of Medicine > Department of Medical Science > 1. Journal Articles
- Graduate School > Department of Biomedical Sciences > 1. Journal Articles
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