Untargeted metabolomics analysis of rat hippocampus subjected to sleep fragmentation
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yoon, Dae Wui | - |
dc.contributor.author | Kwon, Hyuk Nam | - |
dc.contributor.author | Jin, Xing | - |
dc.contributor.author | Kim, Jin Kwan | - |
dc.contributor.author | Lee, Seung Ku | - |
dc.contributor.author | Park, Sunghyouk | - |
dc.contributor.author | Yun, Chang-Ho | - |
dc.contributor.author | Shin, Chol | - |
dc.date.accessioned | 2021-09-01T01:21:05Z | - |
dc.date.available | 2021-09-01T01:21:05Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2019-11 | - |
dc.identifier.issn | 0361-9230 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/62048 | - |
dc.description.abstract | Sleep fragmentation (SF) commonly occurs in several pathologic conditions and is especially associated with impairments of hippocampus-dependent neurocognitive functions. Although the effects of SF on hippocampus in terms of protein or gene levels were examined in several studies, the impact of SF at the metabolite level has not been investigated. Thus, in this study, the differentially expressed large-scale metabolite profiles of hippocampus in a rat model of SF were investigated using untargeted metabolomics approaches. Forty-eight rats were divided into the following 4 groups: 4-day SF group, 4-day exercise control (EC) group, 15-day SF group, and 15-day EC group (n = 12, each). SF was accomplished by forced exercise using a walking wheel system with 30-s on/90-s off cycles, and EC condition was set at 10-min on/30-min off. The metabolite profiles of rat hippocampi in the SF and EC groups were analyzed using liquid chromatography/mass spectrometry. Multivariate analysis revealed distinctive metabolic profiles and marker signals between the SF and corresponding EC groups. Metabolic changes were significant only in the 15-day SF group. In the 15-day SF group, L-tryptophan, myristoylcarnitine, and palmitoylcarnitine were significantly increased, while adenosine monophosphate, hypoxanthine, L-glutamate, L-aspartate, L-methionine, and glycerophosphocholine were decreased compared to the EC group. The alanine, aspartate, and glutamate metabolism pathway was observed as the common key pathway in the 15-day SF groups. The results from this untargeted metabolomics study provide a perspective on metabolic impact of SF on the hippocampus. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | PERGAMON-ELSEVIER SCIENCE LTD | - |
dc.subject | EXCESSIVE DAYTIME SLEEPINESS | - |
dc.subject | SYNAPTIC-TRANSMISSION | - |
dc.subject | CHOLINE DEPRIVATION | - |
dc.subject | GENE-EXPRESSION | - |
dc.subject | PLASTICITY | - |
dc.subject | ASPARTATE | - |
dc.subject | ACETYLCHOLINESTERASE | - |
dc.subject | INCREASES | - |
dc.subject | MELATONIN | - |
dc.subject | DURATION | - |
dc.title | Untargeted metabolomics analysis of rat hippocampus subjected to sleep fragmentation | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Shin, Chol | - |
dc.identifier.doi | 10.1016/j.brainresbull.2019.08.008 | - |
dc.identifier.scopusid | 2-s2.0-85070866671 | - |
dc.identifier.wosid | 000498330200009 | - |
dc.identifier.bibliographicCitation | BRAIN RESEARCH BULLETIN, v.153, pp.74 - 83 | - |
dc.relation.isPartOf | BRAIN RESEARCH BULLETIN | - |
dc.citation.title | BRAIN RESEARCH BULLETIN | - |
dc.citation.volume | 153 | - |
dc.citation.startPage | 74 | - |
dc.citation.endPage | 83 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Neurosciences & Neurology | - |
dc.relation.journalWebOfScienceCategory | Neurosciences | - |
dc.subject.keywordPlus | EXCESSIVE DAYTIME SLEEPINESS | - |
dc.subject.keywordPlus | SYNAPTIC-TRANSMISSION | - |
dc.subject.keywordPlus | CHOLINE DEPRIVATION | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | PLASTICITY | - |
dc.subject.keywordPlus | ASPARTATE | - |
dc.subject.keywordPlus | ACETYLCHOLINESTERASE | - |
dc.subject.keywordPlus | INCREASES | - |
dc.subject.keywordPlus | MELATONIN | - |
dc.subject.keywordPlus | DURATION | - |
dc.subject.keywordAuthor | Sleep fragmentation | - |
dc.subject.keywordAuthor | Hippocampus | - |
dc.subject.keywordAuthor | Untargeted metabolomics | - |
dc.subject.keywordAuthor | Exercise | - |
dc.subject.keywordAuthor | Control | - |
dc.subject.keywordAuthor | Metabolites | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.