Viral antigen nanoparticles for discriminated and quantitative detection of different subtypes of anti-virus immunoglobulins
DC Field | Value | Language |
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dc.contributor.author | Kwon, J-H. | - |
dc.contributor.author | Kim, H-H. | - |
dc.contributor.author | Cho, H-B. | - |
dc.contributor.author | Cha, Y. J. | - |
dc.contributor.author | Lee, J. | - |
dc.date.accessioned | 2021-09-01T01:54:03Z | - |
dc.date.available | 2021-09-01T01:54:03Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2019-10-21 | - |
dc.identifier.issn | 2040-3364 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/62181 | - |
dc.description.abstract | The aim of this study is to develop a novel method for the accurate diagnosis of the infection status of viral diseases, which requires discriminated and quantitative detection of different anti-virus immunoglubulin subtypes. Considering hepatitis A as a representative model disease, viral antigen nanoparticles (vAgNPs) were designed and synthesized by genetically presenting hepatitis A virus (HAV) antigens on the surface of human heavy chain ferritin (hFTH) nanoparticles to detect anti-HAV antibodies with discriminating immunoglobulin subtypes M and G (IgM and IgG, respectively). The vAgNPs also display multi-copies of hexa-histidine peptide (H-6) on their surface to chemisorb gold ions (Au3+), which is vital for the autonomous generation of quantitatively meaningful detection signals. The quantitative level of anti-HAV IgM or IgG in 30 patient sera was successfully analyzed using the vAgNPs of HAV, which was performed through label-free one-step-immunoassay based on the self-enhancement of optical signals from gold nanoparticles clustered on the viral antigen nanoparticles. The diagnostic performance was compared with that of enzyme-linked immunosorbent assay (ELISA), which did not enable accurate quantitative assay due to the poor linearity between the antibody concentration and detection signal. Furthermore, these vAgNP-based immunoassays did not produce any false negative/positive signals, indicating 100% sensitivity and 100% specificity. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ROYAL SOC CHEMISTRY | - |
dc.subject | LINKED-IMMUNOSORBENT-ASSAY | - |
dc.subject | HEPATITIS-A VIRUS | - |
dc.subject | LATERAL FLOW ASSAY | - |
dc.subject | DENGUE VIRUS | - |
dc.subject | GOLD NANOPARTICLES | - |
dc.subject | ACUTE-PHASE | - |
dc.subject | DIAGNOSIS | - |
dc.subject | ANTIBODIES | - |
dc.subject | DISEASE | - |
dc.subject | ENHANCEMENT | - |
dc.title | Viral antigen nanoparticles for discriminated and quantitative detection of different subtypes of anti-virus immunoglobulins | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, J. | - |
dc.identifier.doi | 10.1039/c9nr06160f | - |
dc.identifier.scopusid | 2-s2.0-85073124504 | - |
dc.identifier.wosid | 000512634500036 | - |
dc.identifier.bibliographicCitation | NANOSCALE, v.11, no.39, pp.18282 - 18289 | - |
dc.relation.isPartOf | NANOSCALE | - |
dc.citation.title | NANOSCALE | - |
dc.citation.volume | 11 | - |
dc.citation.number | 39 | - |
dc.citation.startPage | 18282 | - |
dc.citation.endPage | 18289 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalResearchArea | Physics | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Physics, Applied | - |
dc.subject.keywordPlus | LINKED-IMMUNOSORBENT-ASSAY | - |
dc.subject.keywordPlus | HEPATITIS-A VIRUS | - |
dc.subject.keywordPlus | LATERAL FLOW ASSAY | - |
dc.subject.keywordPlus | DENGUE VIRUS | - |
dc.subject.keywordPlus | GOLD NANOPARTICLES | - |
dc.subject.keywordPlus | ACUTE-PHASE | - |
dc.subject.keywordPlus | DIAGNOSIS | - |
dc.subject.keywordPlus | ANTIBODIES | - |
dc.subject.keywordPlus | DISEASE | - |
dc.subject.keywordPlus | ENHANCEMENT | - |
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