Tissue-Clearing Technique and Cutaneous Nerve Biopsies: Quantification of the Intraepidermal Nerve-Fiber Density Using Active Clarity Technique-Pressure Related Efficient and Stable Transfer of Macromolecules Into Organs
DC Field | Value | Language |
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dc.contributor.author | Kim, Dai Hyun | - |
dc.contributor.author | Lee, Se Jeong | - |
dc.contributor.author | Lee, Eunsoo | - |
dc.contributor.author | Hong, Ji Hyuck | - |
dc.contributor.author | Seo, Soo Hong | - |
dc.contributor.author | Ahn, Hyo Hyun | - |
dc.contributor.author | Kim, Byung-Jo | - |
dc.contributor.author | Sun, Woong | - |
dc.contributor.author | Rhyu, Im Joo | - |
dc.date.accessioned | 2021-09-01T05:01:28Z | - |
dc.date.available | 2021-09-01T05:01:28Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2019-10 | - |
dc.identifier.issn | 1738-6586 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/62696 | - |
dc.description.abstract | Background and Purpose Cutaneous nerve biopsies based on two-dimensional analysis have been regarded as a creditable assessment tool for diagnosing peripheral neuropathies. However, advancements in methodological imaging are required for the analysis of intact structures of peripheral nerve fibers. A tissue-clearing and labeling technique facilitates three-dimensional imaging of internal structures in unsectioned, whole biological tissues without excessive time or labor costs. We sought to establish whether a tissue-clearing and labeling technique could be used for the diagnostic evaluation of peripheral neuropathies. Methods Five healthy individuals and four patients with small-fiber neuropathy (SFN) and postherpetic neuralgia (PHN) were prospectively enrolled. The conventional methods of indirect immunofluorescence (IF) and bright-field immunohistochemistry (IHC) were adopted in addition to the tissue-clearing and labeling method called active clarity technique-pressure related efficient and stable transfer of macromolecules into organs (ACT-PRESTO) to quantify the intraepidermal nerve-fiber density (IENFD). Results The mean IENFD values obtained by IF, bright-field IHC, and ACT-PRESTO in the healthy control group were 6.54, 6.44, and 90.19 fibers/mm(2), respectively; the corresponding values in the patients with SFN were 1.99, 2.32, and 48.12 fibers/mm(2), respectively, and 3.06, 2.87, and 47.21 fibers/mm(2), respectively, in the patients with PHN. Conclusions This study has shown that a tissue-clearing method provided not only rapid and highly reproducible three-dimensional images of cutaneous nerve fibers but also yielded reliable quantitative IENFD data. Quantification of the IENFD using a tissue-clearing and labeling technique is a promising way to improve conventional cutaneous nerve biopsies. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | KOREAN NEUROLOGICAL ASSOC | - |
dc.subject | SKIN BIOPSY | - |
dc.subject | INNERVATION | - |
dc.subject | DIAGNOSIS | - |
dc.title | Tissue-Clearing Technique and Cutaneous Nerve Biopsies: Quantification of the Intraepidermal Nerve-Fiber Density Using Active Clarity Technique-Pressure Related Efficient and Stable Transfer of Macromolecules Into Organs | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Seo, Soo Hong | - |
dc.contributor.affiliatedAuthor | Ahn, Hyo Hyun | - |
dc.contributor.affiliatedAuthor | Kim, Byung-Jo | - |
dc.contributor.affiliatedAuthor | Sun, Woong | - |
dc.contributor.affiliatedAuthor | Rhyu, Im Joo | - |
dc.identifier.doi | 10.3988/jcn.2019.15.4.537 | - |
dc.identifier.scopusid | 2-s2.0-85073514152 | - |
dc.identifier.wosid | 000491217300015 | - |
dc.identifier.bibliographicCitation | JOURNAL OF CLINICAL NEUROLOGY, v.15, no.4, pp.537 - 544 | - |
dc.relation.isPartOf | JOURNAL OF CLINICAL NEUROLOGY | - |
dc.citation.title | JOURNAL OF CLINICAL NEUROLOGY | - |
dc.citation.volume | 15 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 537 | - |
dc.citation.endPage | 544 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART002510577 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Neurosciences & Neurology | - |
dc.relation.journalWebOfScienceCategory | Clinical Neurology | - |
dc.subject.keywordPlus | SKIN BIOPSY | - |
dc.subject.keywordPlus | INNERVATION | - |
dc.subject.keywordPlus | DIAGNOSIS | - |
dc.subject.keywordAuthor | cutaneous nerve biopsy | - |
dc.subject.keywordAuthor | peripheral neuropathy | - |
dc.subject.keywordAuthor | tissue-clearing and labeling technique | - |
dc.subject.keywordAuthor | ACT-PRESTO | - |
dc.subject.keywordAuthor | intraepidermal nerve-fiber density | - |
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