Mst1-Deficiency Induces Hyperactivation of Monocyte-Derived Dendritic Cells via Akt1/c-myc Pathway
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Cho, Kyung-Min | - |
dc.contributor.author | Kim, Myun Soo | - |
dc.contributor.author | Jung, Hak-Jun | - |
dc.contributor.author | Choi, Eui-Ju | - |
dc.contributor.author | Kim, Tae Sung | - |
dc.date.accessioned | 2021-09-01T06:15:21Z | - |
dc.date.available | 2021-09-01T06:15:21Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2019-09-11 | - |
dc.identifier.issn | 1664-3224 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/62882 | - |
dc.description.abstract | Mst1 is a multifunctional serine/threonine kinase that is highly expressed in several immune organs. The role of Mst1 in the activation of dendritic cells (DCs), a key player of adaptive immunity, is poorly understood. In this study, we investigated the role of Mst1 in GM-CSF-induced bone marrow-derived DCs and the underlying mechanisms. Mst1(-/-) DCs in response to GM-CSF expressed higher levels of activation/maturation-related cell surface molecules, such as B7 and MHC class II than Mst1(+/+) DCs. Furthermore, the expression of proinflammatory cytokines, such as IL-23, TNF-alpha, and IL-12p40, was increased in Ms1(-/-) DCs, indicating that Mst1-deficiency may induce the hyperactivation of DCs. Additionally, Mst1(-/-) DCs exhibited a stronger capacity to activate allogeneic T cells than Mst1(+/+) DCs. Silencing of Mst1 in DCs promoted their hyperactivation, similar to the phenotypes of Mst1(-/-) DCs. Mst1(-/-) DCs exhibited an increase in Akt1 phosphorylation and c-myc protein levels. In addition, treatment with an Akt1 inhibitor downregulated the protein level of c-myc increased in Mst1-deficient DCs, indicating that Akt1 acts as an upstream inducer of the de novo synthesis of c-myc. Finally, Akt1 and c-myc inhibitors downregulated the increased expression of IL-23p19 observed in Mst1-knockdown DCs. Taken together, these data demonstrate that Mst1 negatively regulates the hyperactivation of DCs through downregulation of the Akt1/c-myc axis in response to GM-CSF, and suggest that Mst1 is one of the endogenous factors that determine the activation status of GM-CSF-stimulated inflammatory DCs. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | FRONTIERS MEDIA SA | - |
dc.subject | REGULATORY T-CELLS | - |
dc.subject | GM-CSF | - |
dc.subject | CROSS-PRESENTATION | - |
dc.subject | OXIDATIVE-STRESS | - |
dc.subject | HIPPO PATHWAY | - |
dc.subject | MST1 | - |
dc.subject | RECEPTOR | - |
dc.subject | PROLIFERATION | - |
dc.subject | MATURATION | - |
dc.subject | INFLAMMATION | - |
dc.title | Mst1-Deficiency Induces Hyperactivation of Monocyte-Derived Dendritic Cells via Akt1/c-myc Pathway | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Choi, Eui-Ju | - |
dc.contributor.affiliatedAuthor | Kim, Tae Sung | - |
dc.identifier.doi | 10.3389/fimmu.2019.02142 | - |
dc.identifier.scopusid | 2-s2.0-85072760925 | - |
dc.identifier.wosid | 000485180600001 | - |
dc.identifier.bibliographicCitation | FRONTIERS IN IMMUNOLOGY, v.10 | - |
dc.relation.isPartOf | FRONTIERS IN IMMUNOLOGY | - |
dc.citation.title | FRONTIERS IN IMMUNOLOGY | - |
dc.citation.volume | 10 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Immunology | - |
dc.relation.journalWebOfScienceCategory | Immunology | - |
dc.subject.keywordPlus | REGULATORY T-CELLS | - |
dc.subject.keywordPlus | GM-CSF | - |
dc.subject.keywordPlus | CROSS-PRESENTATION | - |
dc.subject.keywordPlus | OXIDATIVE-STRESS | - |
dc.subject.keywordPlus | HIPPO PATHWAY | - |
dc.subject.keywordPlus | MST1 | - |
dc.subject.keywordPlus | RECEPTOR | - |
dc.subject.keywordPlus | PROLIFERATION | - |
dc.subject.keywordPlus | MATURATION | - |
dc.subject.keywordPlus | INFLAMMATION | - |
dc.subject.keywordAuthor | Mst1 | - |
dc.subject.keywordAuthor | dendritic cells | - |
dc.subject.keywordAuthor | hyperactivation | - |
dc.subject.keywordAuthor | Akt1 | - |
dc.subject.keywordAuthor | c-myc | - |
dc.subject.keywordAuthor | GM-CSF | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.