Fabrication of bacterial cellulose-collagen composite scaffolds and their osteogenic effect on human mesenchymal stem cells
- Authors
- Noh, Yong Kwan; Da Costa, Avelino Dos Santos; Park, Yong Seek; Du, Ping; Kim, Ik-Hwan; Park, Kwideok
- Issue Date
- 1-9월-2019
- Publisher
- ELSEVIER SCI LTD
- Keywords
- Bacterial cellulose (BC); Collagen; Umbilical cord blood derived mesenchymal stem cells (UCB-MSCs); Osteogenesis; Bone regeneration
- Citation
- CARBOHYDRATE POLYMERS, v.219, pp.210 - 218
- Indexed
- SCIE
SCOPUS
- Journal Title
- CARBOHYDRATE POLYMERS
- Volume
- 219
- Start Page
- 210
- End Page
- 218
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/62939
- DOI
- 10.1016/j.carbpol.2019.05.039
- ISSN
- 0144-8617
- Abstract
- Scaffold plays a critical role in stem cell differentiation and tissue regeneration. Composite scaffolds composed of bacterial cellulose (BC) and collagen (Col) in different ratios (1:1, 3:1, 5:1) were fabricated in this study. The composite scaffolds exhibit a well-organized interconnected porous structure, significantly better physical stability than Col scaffold, and more water uptake up to 400%. They were also favorable with cell attachment and growth. After osteogenic induction of umbilical cord blood derived mesenchymal stem cells (UCB-MSCs) for 3 weeks, we found more up-regulated osteogenic markers (collagen type 1, osteocalcin, bone sialoprotein) and significantly elevated proteins and calcium deposition, particularly with BC/Col (5:1) scaffold. When PKH-26 pre-labelled MSC-loaded scaffolds were subcutaneously transplanted in a mouse model, they showed many PKH-26-labelled cells and positive signals of a-smooth muscle actin, for neovascularization in the BC/Col (5:1). The current work demonstrates that our BC/Col composites may be promising as a bone tissue-engineered scaffold.
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Collections - College of Life Sciences and Biotechnology > Division of Life Sciences > 1. Journal Articles
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