Identification of different gene expressions between diffuse- and intestinal-type spheroid-forming gastric cancer cells
DC Field | Value | Language |
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dc.contributor.author | Lee, Jong Won | - |
dc.contributor.author | Sung, Jae Sook | - |
dc.contributor.author | Park, Young Soo | - |
dc.contributor.author | Chung, Seok | - |
dc.contributor.author | Kim, Yeul Hong | - |
dc.date.accessioned | 2021-09-01T07:21:23Z | - |
dc.date.available | 2021-09-01T07:21:23Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2019-09 | - |
dc.identifier.issn | 1436-3291 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/62993 | - |
dc.description.abstract | Background Three-dimensional in vitro spheroid models are unique because they are considered for enrichment of specific cell populations with self-renewal ability. In this study, we explored the different mechanisms of gastric cancer spheroid-forming cells according to the Lauren classification. Methods We isolated and enriched cells with self-renewal ability using spheroid-forming methods from gastric cancer cell lines. The expression of candidate target genes was investigated using western blot and qRT-PCR analysis. Lentiviral shRNA knockdown of target gene expression was performed and the effects on spheroid, colony forming, and tumorigenic ability were analyzed. Results The SNU-638, SNU-484, MKN-28, and NCI-N87 successfully formed spheroid from single cell and enriched for self-renewal ability from 11 gastric cancer cell lines, including diffuse and intestinal types. The expression of SOX2 and E-cadherin increased in spheroid-forming cells in a diffuse-type cell line (SNU-638 and SNU-484), but not in the intestinal type (MKN-28 and NCI-N87). In contrast, ERBB3 expression was only increased in intestinal-type spheroid cells. The depletion of each candidate target gene expression suppressed self-renewal ability to grow as spheroids and colonies in a soft agar assay. In particular, down-regulated ERBB3 in the intestinal-type cell lines inhibited tumor growth in a mouse xenograft model. We found that high ERBB3 gene expression correlates with decreased survival in the intestinal type of gastric cancer. Conclusions Our results suggest that diffuse- and intestinal-type spheroid-forming cells express genes differently. Our data suggest that these candidate genes from spheroid-forming cells can be used in applications in targeted therapy. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | SPRINGER | - |
dc.subject | E-CADHERIN | - |
dc.subject | SURVIVAL | - |
dc.subject | MARKER | - |
dc.subject | GROWTH | - |
dc.subject | TOOL | - |
dc.title | Identification of different gene expressions between diffuse- and intestinal-type spheroid-forming gastric cancer cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Jong Won | - |
dc.contributor.affiliatedAuthor | Chung, Seok | - |
dc.contributor.affiliatedAuthor | Kim, Yeul Hong | - |
dc.identifier.doi | 10.1007/s10120-019-00935-x | - |
dc.identifier.scopusid | 2-s2.0-85061214026 | - |
dc.identifier.wosid | 000481429600006 | - |
dc.identifier.bibliographicCitation | GASTRIC CANCER, v.22, no.5, pp.967 - 979 | - |
dc.relation.isPartOf | GASTRIC CANCER | - |
dc.citation.title | GASTRIC CANCER | - |
dc.citation.volume | 22 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 967 | - |
dc.citation.endPage | 979 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Oncology | - |
dc.relation.journalResearchArea | Gastroenterology & Hepatology | - |
dc.relation.journalWebOfScienceCategory | Oncology | - |
dc.relation.journalWebOfScienceCategory | Gastroenterology & Hepatology | - |
dc.subject.keywordPlus | E-CADHERIN | - |
dc.subject.keywordPlus | SURVIVAL | - |
dc.subject.keywordPlus | MARKER | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordPlus | TOOL | - |
dc.subject.keywordAuthor | Gastric cancer | - |
dc.subject.keywordAuthor | Cellular spheroid | - |
dc.subject.keywordAuthor | Lauren classification | - |
dc.subject.keywordAuthor | Diffuse type | - |
dc.subject.keywordAuthor | Intestinal type | - |
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