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Development of a new 15-valent pneumococcal conjugate vaccine (PCV15) and evaluation of its immunogenicity

Authors
Lee, ChankyuChoi, Seuk KeunKim, Rock KiKim, HeeyounWhang, Yoon HeePharm, HuyenCheon, HyunwooYoon, Do-YoungKim, Chan WhaBaik, Yeong OkPark, Sung SooLee, Inhwan
Issue Date
9월-2019
Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Keywords
Polyvalent pneumococcal conjugate vaccine; Immunogenicity; Enzyme-linked immunosorbent assay; Opsonophagocytic activity
Citation
BIOLOGICALS, v.61, pp.32 - 37
Indexed
SCIE
SCOPUS
Journal Title
BIOLOGICALS
Volume
61
Start Page
32
End Page
37
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/63078
DOI
10.1016/j.biologicals.2019.07.005
ISSN
1045-1056
Abstract
A new 15-valent pneumococcal conjugate vaccine (PCV15) against serotypes 1, 3, 4, 5, 6A, 6B, 7F, 9V, 11A, 14, 18C, 19A, 19F, 22F, and 23F has been developed using aluminum phosphate as an adjuvant. Using the rabbit model, immunogenicity of each serotype was evaluated by measuring antigen specific antibodies and functional antibody titers and comparing them to a control vaccine, Prevnar13 (R). Among the shared serotypes in both PCV15 and Prevnar13 (R), Type 3 and 23F in PCV15 exhibited a lower opsonic index than Prevnar13 (R). Conversely, the other types showed greater or nearly the same immunogenic effects. Type 11A and 22F are two additional serotypes included in PCV15, and only 22F showed a reasonable opsonic index compared with other types. Type 11A exhibited a basal level fold-increase in OPA; thus, we further optimized 11A as well as 3 and 23F by controlling the polysaccharide-to-protein conjugation ratio as a variable. Antibody levels and functional antibody activities were evaluated by ELISA and OPA, and improved levels of immunogenic activities were observed for all three serotypes. In this study, we propose a new PCV15 candidate, in which the common 13 serotypes and a licensed control vaccine have equivalent efficacy while two additional serotypes showed adequate immunogenicity in the rabbit model.
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