Metabolite tracking to elucidate the effects of environmental pollutants
- Authors
- Park, Minseung; Lee, Yeseung; Khan, Adnan; Aleta, Prince; Cho, Yunchul; Park, Hongkeun; Park, Youngja Hwang; Kim, Sungpyo
- Issue Date
- 15-8월-2019
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- Toxicity; Zebrafish; Metabolomics
- Citation
- JOURNAL OF HAZARDOUS MATERIALS, v.376, pp.112 - 124
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF HAZARDOUS MATERIALS
- Volume
- 376
- Start Page
- 112
- End Page
- 124
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/63513
- DOI
- 10.1016/j.jhazmat.2019.05.024
- ISSN
- 0304-3894
- Abstract
- The purpose of this study was to determine whether behavioral tests and metabolic profiling of organisms can be promising alternatives for assessing the health of aquatic systems. Water samples from four potential pollution sources in South Korea were collected for toxicity evaluation. First, conventional acute toxicity test in Daphnia magna and behavioral test in zebrafish was conducted to assess water quality. Second, metabolomic analysis was performed on zebrafish exposed to water samples and on environmental fish collected from the same source. Acute toxicity test in D. magna showed that none of the water samples exerted significant adverse effects. However, activity of zebrafish larvae exposed to samples from the zinc smelter (ZS) and industrial complex (IND) sites decreased compared to those exposed to samples from the reference site (RS). Metabolomic analysis using the Manhattan plot and Partial Least Square (PLS)/Orthogonal PLS Discriminant Analysis (OPLS-DA) showed differences in metabolic profiles between RS and ZS, and between IND and abandoned mine site (M). Interestingly, applying the same metabolomic analysis to environmental fish revealed patterns similar to those for zebrafish, despite the uncontrollable variables involved in environmental sampling. This study shows that metabolomics is a promising tool in assessing the health of aquatic environments.
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Collections - College of Pharmacy > Department of Pharmaceutical Science > 1. Journal Articles
- Graduate School > Department of Environmental Engineering > 1. Journal Articles
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