Diagnostic performance of the Xpert Carba-R assay for active surveillance of rectal carbapenemase-producing organisms in intensive care unit patients
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ko, Young Jin | - |
dc.contributor.author | Kim, Jeeyong | - |
dc.contributor.author | Kim, Ha-Nui | - |
dc.contributor.author | Yoon, Soo-Young | - |
dc.contributor.author | Lim, Chae Seung | - |
dc.contributor.author | Lee, Chang Kyu | - |
dc.date.accessioned | 2021-09-01T11:03:59Z | - |
dc.date.available | 2021-09-01T11:03:59Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2019-07-29 | - |
dc.identifier.issn | 2047-2994 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/64053 | - |
dc.description.abstract | BackgroundThere are growing concerns regarding the spread of carbapenemase-producing organisms (CPOs) among patients in long-term care facilities (LTCFs) and hospitals in South Korea. We have established a screening protocol for the detection of CPOs in high-risk patients upon admission to intensive care units (ICUs). The diagnostic performance of the Xpert Carba-R assay was compared to that of rectal culture for CPO detection in high-risk patients upon ICU admission.MethodsA total of 408 consecutive rectal swabs were obtained from December 2016 to December 2017. CPO screening was performed using the Xpert Carba-R assay (Cepheid, Sunnyvale, CA, USA). When a carbapenemase gene was detected, additional rectal swabs were incubated overnight and inoculated on chromID CARBA medium (bioMerieux, Marcy l'Etoile, France). Bacterial carbapenemase genes, including bla(KPC), bla(NDM), bla(VIM), bla(IMP-1), and bla(OXA-48), were confirmed by conventional PCR. The diagnostic performance of the Carba-R assay was ascertained based on the culture results.ResultsThe prevalence of CPO carriage was 7.4% according to the Carba-R assay and 3.7% according to rectal culture. The median Ct values of IMP-1 and KPC were significantly different (35.2 vs. 26.6, P=0.0143). The overall sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of the Carba-R assay were 100.0% (95% confidence interval [CI], 78.2-100.0), 96.7% (94.4-98.2), 53.6% (40.4-66.4) and 100.0% (99.0-100.0), respectively.ConclusionsWe demonstrated the prevalence of CPO carriage in high-risk patients upon ICU admission and evaluated the diagnostic performance of the Carba-R assay. The combined use of the Xpert Carba-R assay and culture produces rapid and reliable results for the active surveillance of rectal CPO in ICU patients. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | BMC | - |
dc.subject | GRAM-NEGATIVE BACILLI | - |
dc.subject | KLEBSIELLA-PNEUMONIAE | - |
dc.subject | MULTIPLEX PCR | - |
dc.subject | ENTEROBACTERIACEAE | - |
dc.subject | EPIDEMIOLOGY | - |
dc.subject | COLONIZATION | - |
dc.subject | RESISTANCE | - |
dc.title | Diagnostic performance of the Xpert Carba-R assay for active surveillance of rectal carbapenemase-producing organisms in intensive care unit patients | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Jeeyong | - |
dc.contributor.affiliatedAuthor | Yoon, Soo-Young | - |
dc.contributor.affiliatedAuthor | Lim, Chae Seung | - |
dc.contributor.affiliatedAuthor | Lee, Chang Kyu | - |
dc.identifier.doi | 10.1186/s13756-019-0579-2 | - |
dc.identifier.scopusid | 2-s2.0-85070065813 | - |
dc.identifier.wosid | 000477806800001 | - |
dc.identifier.bibliographicCitation | ANTIMICROBIAL RESISTANCE AND INFECTION CONTROL, v.8 | - |
dc.relation.isPartOf | ANTIMICROBIAL RESISTANCE AND INFECTION CONTROL | - |
dc.citation.title | ANTIMICROBIAL RESISTANCE AND INFECTION CONTROL | - |
dc.citation.volume | 8 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Public, Environmental & Occupational Health | - |
dc.relation.journalResearchArea | Infectious Diseases | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Public, Environmental & Occupational Health | - |
dc.relation.journalWebOfScienceCategory | Infectious Diseases | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.subject.keywordPlus | GRAM-NEGATIVE BACILLI | - |
dc.subject.keywordPlus | KLEBSIELLA-PNEUMONIAE | - |
dc.subject.keywordPlus | MULTIPLEX PCR | - |
dc.subject.keywordPlus | ENTEROBACTERIACEAE | - |
dc.subject.keywordPlus | EPIDEMIOLOGY | - |
dc.subject.keywordPlus | COLONIZATION | - |
dc.subject.keywordPlus | RESISTANCE | - |
dc.subject.keywordAuthor | Carbapenemase | - |
dc.subject.keywordAuthor | Surveillance | - |
dc.subject.keywordAuthor | Xpert Carba-R assay | - |
dc.subject.keywordAuthor | Gram-negative bacteria | - |
dc.subject.keywordAuthor | Intensive care unit | - |
dc.subject.keywordAuthor | Molecular methods | - |
dc.subject.keywordAuthor | Chromogenic culture | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
145 Anam-ro, Seongbuk-gu, Seoul, 02841, Korea+82-2-3290-2963
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.