Overexpression of Nanog in amniotic fluid-derived mesenchymal stem cells accelerates dermal papilla cell activity and promotes hair follicle regeneration
DC Field | Value | Language |
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dc.contributor.author | Park, Junghyun | - |
dc.contributor.author | Jun, Eun Kyoung | - |
dc.contributor.author | Son, Daryeon | - |
dc.contributor.author | Hong, Wonjun | - |
dc.contributor.author | Jang, Jihoon | - |
dc.contributor.author | Yun, Wonjin | - |
dc.contributor.author | Yoon, Byung Sun | - |
dc.contributor.author | Song, Gwonhwa | - |
dc.contributor.author | Kim, In Yong | - |
dc.contributor.author | You, Seungkwon | - |
dc.date.accessioned | 2021-09-01T12:03:55Z | - |
dc.date.available | 2021-09-01T12:03:55Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2019-07-04 | - |
dc.identifier.issn | 1226-3613 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/64141 | - |
dc.description.abstract | Alopecia, one of the most common chronic diseases, can seriously affect a patient's psychosocial life. Dermal papilla (DP) cells serve as essential signaling centers in the regulation of hair growth and regeneration and are associated with crosstalk between autocrine/paracrine factors and the surrounding environment. We previously demonstrated that amniotic fluid-derived mesenchymal stem cell-conditioned medium (AF-MSC-CM) accelerates hair regeneration and growth. The present study describes the effects of overexpression of a reprogramming factor, Nanog, on MSC properties, the paracrine effects on DP cells, and in vivo hair regrowth. First, we examined the in vitro proliferation and lifespan of AF-MSCs overexpressing reprogramming factors, including Oct4, Nanog, and Lin28, alone or in combination. Among these factors, Nanog was identified as a key factor in maintaining the self-renewal capability of AF-MSCs by delaying cellular senescence, increasing the endogenous expression of Oct4 and Sox2, and preserving stemness. Next, we evaluated the paracrine effects of AF-MSCs overexpressing Nanog (AF-N-MSCs) by monitoring secretory molecules related to hair regeneration and growth (IGF, PDGF, bFGF, and Wnt7a) and proliferation of DP cells. In vivo studies revealed that CM derived from AF-N-MSCs (AF-N-CM) accelerated the telogen-to-anagen transition in hair follicles (HFs) and increased HF density. The expression of DP and HF stem cell markers and genes related to hair induction were higher in AF-N-CM than in CM from AF-MSCs (AF-CM). This study suggests that the secretome from autologous MSCs overexpressing Nanog could be an excellent candidate as a powerful anagen inducer and hair growth stimulator for the treatment of alopecia. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.subject | DNA-DAMAGE RESPONSE | - |
dc.subject | GROWTH-FACTOR | - |
dc.subject | ANDROGENETIC ALOPECIA | - |
dc.subject | SELF-RENEWAL | - |
dc.subject | ANAGEN PHASE | - |
dc.subject | DIFFERENTIATION | - |
dc.subject | EXPRESSION | - |
dc.subject | PLURIPOTENCY | - |
dc.subject | APOPTOSIS | - |
dc.subject | CYCLE | - |
dc.title | Overexpression of Nanog in amniotic fluid-derived mesenchymal stem cells accelerates dermal papilla cell activity and promotes hair follicle regeneration | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Song, Gwonhwa | - |
dc.contributor.affiliatedAuthor | You, Seungkwon | - |
dc.identifier.doi | 10.1038/s12276-019-0266-7 | - |
dc.identifier.scopusid | 2-s2.0-85068479609 | - |
dc.identifier.wosid | 000474510800002 | - |
dc.identifier.bibliographicCitation | EXPERIMENTAL AND MOLECULAR MEDICINE, v.51 | - |
dc.relation.isPartOf | EXPERIMENTAL AND MOLECULAR MEDICINE | - |
dc.citation.title | EXPERIMENTAL AND MOLECULAR MEDICINE | - |
dc.citation.volume | 51 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.subject.keywordPlus | DNA-DAMAGE RESPONSE | - |
dc.subject.keywordPlus | GROWTH-FACTOR | - |
dc.subject.keywordPlus | ANDROGENETIC ALOPECIA | - |
dc.subject.keywordPlus | SELF-RENEWAL | - |
dc.subject.keywordPlus | ANAGEN PHASE | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | PLURIPOTENCY | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.subject.keywordPlus | CYCLE | - |
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