Cohnella candidum sp. nov., radiation-resistant bacterium from soil

Abstract

A Gram-stain positive, endospore-forming, circular, convex, cream colored, designated strain 18JY8-7(T), was isolated from soil collected in Jeju Island, South Korea. Phylogenetic analysis using 16S rRNA gene sequences showed that strain 18JY8-7(T) formed a distinct lineage within the family Paenibacillaceae (order Bacillales, class Bacilli), and is closely related to Cohnella rhizosphaerae (96.1%, sequence similarity) and Cohnella xylanilytica (96.0%). Optimal growth occurred at 30 degrees C, pH 6.5 and in the absence of NaCl. The predominant cellular fatty acids were anteiso-C-15:0 and iso-C-16:0. The major respiratory quinone was MK-7. The polar lipids profile comprised of diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine. The DNA G+C content was 57.0mol%. The genotypic and phenotypic analyses revealed the differentiation of strain 18JY8-7(T) from all recognized Cohenella species. The strain 18JY8-7(T), therefore represents a novel bacterial species within the family Paenibacillaceae, for which the name Cohnella candidum sp. nov. is proposed. The type strain is 18JY8-7(T) (=KCTC 33969(T)=JCM 33199(T)).