Enhanced In-Vitro Hemozoin Polymerization by Optimized Process using Histidine-Rich Protein II (HRPII)
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Ju Hun | - |
dc.contributor.author | Kim, Hyeong Ryeol | - |
dc.contributor.author | Lee, Ja Hyun | - |
dc.contributor.author | Lee, Soo Kweon | - |
dc.contributor.author | Chun, Youngsang | - |
dc.contributor.author | Han, Sung Ok | - |
dc.contributor.author | Yoo, Hah Young | - |
dc.contributor.author | Park, Chulhwan | - |
dc.contributor.author | Kim, Seung Wook | - |
dc.date.accessioned | 2021-09-01T13:30:41Z | - |
dc.date.available | 2021-09-01T13:30:41Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2019-07 | - |
dc.identifier.issn | 2073-4360 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/64667 | - |
dc.description.abstract | Conductive biopolymers, an important class of functional materials, have received attention in various fields because of their unique electrical, optical, and physical properties. In this study, the polymerization of heme into hemozoin was carried out in an in vitro system by the newly developed heme polymerase (histidine-rich protein 2 (HRP-II)). The HRP-II was produced by recombinant E. coli BL21 from the Plasmodium falciparum gene. To improve the hemozoin production, the reaction conditions on the polymerization were investigated and the maximum production was achieved after about 790 mu M at 34 degrees C with 200 rpm for 24 h. As a result, the production was improved about two-fold according to the stepwise optimization in an in vitro system. The produced hemozoin was qualitatively analyzed using the Fourier transform infrared (FTIR) spectroscopy, energy dispersive X-ray spectroscopy (EDS), and scanning electron microscopy (SEM). Finally, it was confirmed that the enzymatically polymerized hemozoin had similar physical properties to chemically synthesized hemozoin. These results could represent a significant potential for nano-biotechnology applications, and also provide guidance in research related to hemozoin utilization. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.subject | PLASMODIUM-FALCIPARUM | - |
dc.subject | HEMATIN FORMATION | - |
dc.subject | HEME-PROTEINS | - |
dc.subject | MALARIA | - |
dc.subject | PERFORMANCE | - |
dc.subject | POLYMERS | - |
dc.subject | IRON | - |
dc.title | Enhanced In-Vitro Hemozoin Polymerization by Optimized Process using Histidine-Rich Protein II (HRPII) | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Han, Sung Ok | - |
dc.contributor.affiliatedAuthor | Kim, Seung Wook | - |
dc.identifier.doi | 10.3390/polym11071162 | - |
dc.identifier.scopusid | 2-s2.0-85070350392 | - |
dc.identifier.wosid | 000480539500079 | - |
dc.identifier.bibliographicCitation | POLYMERS, v.11, no.7 | - |
dc.relation.isPartOf | POLYMERS | - |
dc.citation.title | POLYMERS | - |
dc.citation.volume | 11 | - |
dc.citation.number | 7 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Polymer Science | - |
dc.relation.journalWebOfScienceCategory | Polymer Science | - |
dc.subject.keywordPlus | PLASMODIUM-FALCIPARUM | - |
dc.subject.keywordPlus | HEMATIN FORMATION | - |
dc.subject.keywordPlus | HEME-PROTEINS | - |
dc.subject.keywordPlus | MALARIA | - |
dc.subject.keywordPlus | PERFORMANCE | - |
dc.subject.keywordPlus | POLYMERS | - |
dc.subject.keywordPlus | IRON | - |
dc.subject.keywordAuthor | biopolymers | - |
dc.subject.keywordAuthor | heme | - |
dc.subject.keywordAuthor | hemozoin | - |
dc.subject.keywordAuthor | HRP-II | - |
dc.subject.keywordAuthor | polymerase | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.