Generation and characterization of a monoclonal antibody against MERS-CoV targeting the spike protein using a synthetic peptide epitope-CpG-DNA-liposome complex
DC Field | Value | Language |
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dc.contributor.author | Park, Byoung Kwon | - |
dc.contributor.author | Maharjan, Sony | - |
dc.contributor.author | Lee, Su In | - |
dc.contributor.author | Kim, Jinsoo | - |
dc.contributor.author | Bae, Joon-Yong | - |
dc.contributor.author | Park, Man-Seong | - |
dc.contributor.author | Kwon, Hyung-Joo | - |
dc.date.accessioned | 2021-09-01T13:36:06Z | - |
dc.date.available | 2021-09-01T13:36:06Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2019-06-30 | - |
dc.identifier.issn | 1976-6696 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/64717 | - |
dc.description.abstract | Middle East respiratory syndrome coronavirus (MERS-CoV) uses the spike (S) glycoprotein to recognize and enter target cells. In this study, we selected two epitope peptide sequences within the receptor binding domain (RBD) of the MERS-CoV S protein. We used a complex consisting of the epitope peptide of the MERS-CoV S protein and CpG-DNA encapsulated in liposome complex to immunize mice, and produced the monoclonal antibodies 506-2G10G5 and 492-1G10E4E2. The western blotting data showed that both monoclonal antibodies detected the S protein and immunoprecipitated the native form of the S protein. Indirect immunofluorescence and confocal analysis suggested strong reactivity of the antibodies towards the S protein of MERS-CoV virus infected Vero cells. Furthermore, the 506-2G10G5 monoclonal antibody significantly reduced plaque formation in MERS-CoV infected Vero cells compared to normal mouse IgG and 492-1G10E4E2. Thus, we successfully produced a monoclonal antibody directed against the RBD domain of the S protein which could be used in the development of diagnostics and therapeutic applications in the future. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | KOREAN SOCIETY BIOCHEMISTRY & MOLECULAR BIOLOGY | - |
dc.subject | CORONAVIRUS | - |
dc.subject | RECEPTOR | - |
dc.title | Generation and characterization of a monoclonal antibody against MERS-CoV targeting the spike protein using a synthetic peptide epitope-CpG-DNA-liposome complex | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Park, Man-Seong | - |
dc.identifier.doi | 10.5483/BMBRep.2019.52.6.185 | - |
dc.identifier.scopusid | 2-s2.0-85068991871 | - |
dc.identifier.wosid | 000473211900007 | - |
dc.identifier.bibliographicCitation | BMB REPORTS, v.52, no.6, pp.397 - 402 | - |
dc.relation.isPartOf | BMB REPORTS | - |
dc.citation.title | BMB REPORTS | - |
dc.citation.volume | 52 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 397 | - |
dc.citation.endPage | 402 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART002475755 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.subject.keywordPlus | CORONAVIRUS | - |
dc.subject.keywordPlus | RECEPTOR | - |
dc.subject.keywordAuthor | B cell epitope | - |
dc.subject.keywordAuthor | Lipoplex (O) | - |
dc.subject.keywordAuthor | MERS-CoV | - |
dc.subject.keywordAuthor | Monoclonal antibody | - |
dc.subject.keywordAuthor | Spike protein | - |
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