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Permselective glucose sensing with GLUT1-rich cancer cell membranes

Authors
Kim, InsuKwon, DohyungLee, DongtakLee, GyudoYoon, Dae Sung
Issue Date
15-6월-2019
Publisher
ELSEVIER ADVANCED TECHNOLOGY
Keywords
Electrochemical analysis; Glucose biosensor; Cancer cell membrane; Glucose transporter-1; Permselectivity
Citation
BIOSENSORS & BIOELECTRONICS, v.135, pp.82 - 87
Indexed
SCIE
SCOPUS
Journal Title
BIOSENSORS & BIOELECTRONICS
Volume
135
Start Page
82
End Page
87
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/64765
DOI
10.1016/j.bios.2019.04.007
ISSN
0956-5663
Abstract
Enzymatic blood glucose detection with selectivity is one of the most important conundrums, because human blood contains many components that can hinder enzyme-substrate reactions. Meanwhile, cancer cells express much higher levels of glucose transporter-1 on their cell membrane to selectively and excessively uptake more alpha-D-glucose than do normal cells. Inspired by such cellular permselectivity for glucose, herein we significantly improved the selectivity of a glucose sensor by using a breast cancer cell membrane (BCCM). The BCCM was extracted from MDA-MB-231 cells and coated onto an enzyme-deposited electrode via a vesicle fusion method. We investigated BCCM-coated sensors using ATR-FTIR, SEM, AFM, and cyclic voltammetry. The exceptional permselectivity of BCCM-coated sensors was validated using glucose solutions containing various interfering molecules (e.g., D-( - )-fructose, D-( + )-xylose, D-( + )-maltose, L-cysteine, L-ascorbic acid, and uric acid) and human serum (4.35-7.35 mM of glucose), implying their high potential for practical use.
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Graduate School > Department of Biotechnology and Bioinformatics > 1. Journal Articles
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