Generation of Anterior Hindbrain-Specific, Glial-Restricted Progenitor-Like Cells from Human Pluripotent Stem Cells
- Authors
- Yun, Wonjin; Hong, Wonjun; Son, Daryeon; Liu, Hui-Wen; Kim, Seung-Soo; Park, Minji; Kim, In Yong; Kim, Dae-Sung; Song, Gwonhwa; You, Seungkwon
- Issue Date
- 15-5월-2019
- Publisher
- MARY ANN LIEBERT, INC
- Keywords
- glial restricted progenitor cells; astrocytes; oligodendrocytes; differentiation; hindbrain
- Citation
- STEM CELLS AND DEVELOPMENT, v.28, no.10, pp.633 - 648
- Indexed
- SCIE
SCOPUS
- Journal Title
- STEM CELLS AND DEVELOPMENT
- Volume
- 28
- Number
- 10
- Start Page
- 633
- End Page
- 648
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/65416
- DOI
- 10.1089/scd.2019.0033
- ISSN
- 1547-3287
- Abstract
- Engraftment of oligodendrocyte progenitor cells (OPCs), which form myelinating oligodendrocytes, has the potential to treat demyelinating diseases such as multiple sclerosis. However, conventional strategies for generating oligodendrocytes have mainly focused on direct differentiation into forebrain- or spinal cord-restricted oligodendrocytes without establishing or amplifying stem/progenitor cells. Taking advantage of a recently established culture system, we generated expandable EN1- and GBX2-positive glial-restricted progenitor-like cells (GPLCs) near the anterior hindbrain. These cells expressed PDGFR alpha, CD9, S100 beta, and SOX10 and mostly differentiated into GFAP-positive astrocytes and MBP-positive oligodendrocytes. RNA-seq analysis revealed that the transcriptome of GPLCs was similar to that of O4-positive OPCs, but distinct from that of rosette-type neural stem cells. Notably, engrafted GPLCs not only differentiated into GFAP-positive astrocytes but also myelinated the brains of adult shiverer mice 8 weeks after transplantation. Our strategy for establishing anterior hindbrain-specific GPLCs with gliogenic potency will facilitate their use in the treatment of demyelinating diseases and studies of the molecular mechanisms underlying glial development in the hindbrain.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - Graduate School > Department of Biotechnology > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.