Endoribonucleolytic Cleavage of m(6)A-Containing RNAs by RNase P/MRP Complex
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, Ok Hyun | - |
dc.contributor.author | Ha, Hongseok | - |
dc.contributor.author | Lee, Yujin | - |
dc.contributor.author | Boo, Sung Ho | - |
dc.contributor.author | Kwon, Do Hoon | - |
dc.contributor.author | Song, Hyun Kyu | - |
dc.contributor.author | Kim, Yoon Ki | - |
dc.date.accessioned | 2021-09-01T15:08:20Z | - |
dc.date.available | 2021-09-01T15:08:20Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2019-05-02 | - |
dc.identifier.issn | 1097-2765 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/65465 | - |
dc.description.abstract | N-6-methyladenosine (m(6)A) is the most abundant internal modification in RNAs and plays regulatory roles in a variety of biological and physiological processes. Despite its important roles, the molecular mechanism underlying m(6)A-mediated gene regulation is poorly understood. Here, we show that m(6)A-containing RNAs are subject to endoribonucleolytic cleavage via YTHDF2 (m(6)A reader protein), HRSP12 (adaptor protein), and RNase P/MRP (endoribonucleases). We demonstrate that HRSP12 functions as an adaptor to bridge YTHDF2 and RNase P/MRP, eliciting rapid degradation of YTHDF2-bound RNAs. Transcriptome-wide analyses show that m(6)A RNAs that are preferentially targeted for endoribonucleolytic cleavage have an HRSP12-binding site and a RNase P/MRP-directed cleavage site upstream and downstream of the YTHDF2-binding site, respectively. We also find that a subset of m(6)A-containing circular RNAs associates with YTHDF2 in an HRSP12-dependent manner and is selectively downregulated by RNase P/MRP. Thus, our data expand the known functions of RNase P/MRP to endoribonucleolytic cleavage of m(6)A RNAs. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | CELL PRESS | - |
dc.subject | IDENTIFICATION | - |
dc.subject | TRANSLATION | - |
dc.subject | REVEALS | - |
dc.subject | PROTEIN | - |
dc.subject | ROLES | - |
dc.subject | SITES | - |
dc.subject | NMD | - |
dc.title | Endoribonucleolytic Cleavage of m(6)A-Containing RNAs by RNase P/MRP Complex | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Song, Hyun Kyu | - |
dc.contributor.affiliatedAuthor | Kim, Yoon Ki | - |
dc.identifier.doi | 10.1016/j.molcel.2019.02.034 | - |
dc.identifier.scopusid | 2-s2.0-85064865187 | - |
dc.identifier.wosid | 000466703900010 | - |
dc.identifier.bibliographicCitation | MOLECULAR CELL, v.74, no.3, pp.494 - + | - |
dc.relation.isPartOf | MOLECULAR CELL | - |
dc.citation.title | MOLECULAR CELL | - |
dc.citation.volume | 74 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 494 | - |
dc.citation.endPage | + | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | TRANSLATION | - |
dc.subject.keywordPlus | REVEALS | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | ROLES | - |
dc.subject.keywordPlus | SITES | - |
dc.subject.keywordPlus | NMD | - |
dc.subject.keywordAuthor | CCR4-NOT complex | - |
dc.subject.keywordAuthor | circular RNA | - |
dc.subject.keywordAuthor | endoribonucleolytic cleavage | - |
dc.subject.keywordAuthor | FTO | - |
dc.subject.keywordAuthor | HRSP12 | - |
dc.subject.keywordAuthor | m6A modification | - |
dc.subject.keywordAuthor | METTL3 | - |
dc.subject.keywordAuthor | RNA decay | - |
dc.subject.keywordAuthor | RNase P/MRP | - |
dc.subject.keywordAuthor | YTHDF2 | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
145 Anam-ro, Seongbuk-gu, Seoul, 02841, Korea+82-2-3290-2963
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.