Detailed Information

Cited 0 time in webofscience Cited 0 time in scopus
Metadata Downloads

Correlation between the results of two analytical methods for measuring measles virus neutralizing antibodies in source plasma and therapeutic immunoglobulin products

Authors
Hong, JeungwoonKim, DaegeunWon, YounheeYoon, JungsoonPark, Kuk JinOh, JaetaekKim, Chan-Wha
Issue Date
5월-2019
Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Keywords
Intravenous immunoglobulin; Measles neutralizing antibody; mnAbs ELISA; TCND50; Source plasma; Correlation
Citation
BIOLOGICALS, v.59, pp.20 - 28
Indexed
SCIE
SCOPUS
Journal Title
BIOLOGICALS
Volume
59
Start Page
20
End Page
28
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/65906
DOI
10.1016/j.biologicals.2019.03.009
ISSN
1045-1056
Abstract
Patients with primary immunodeficiency disorders are vulnerable to infectious diseases. Intravenous immunoglobulin (IVIG) therapeutic products manufactured from human plasma are employed widely to protect patients from pathogens such as measles virus, which causes a potentially fatal and contagious disease. Therefore, health authorities stipulate a minimum titer of measles neutralizing antibodies (mnAbs) in IVIG products to ensure efficient protection. In general, mnAb titers are measured in a cell-based neutralization assay; however, this assay is labor intensive and time consuming, and the results are variable. Here, we compared a cell-based neutralizing assay with several ELISA tests to evaluate whether ELISAs can overcome the limitations of cell-based assays. The mnAb concentrations measured by the ELISAs showed a strong and significant positive correlation with those measured in a cell-based assay. Also, strong positive correlations were identified for measurement of individual source plasmas, which are used as raw materials for manufacturing IVIG products. Measurement by ELISA revealed that about 80% of 198 source plasmas had mnAb concentrations of < 500 mIU/mL. These results suggest that quantitative ELISAs based on relevant antigens allow reliable and comprehensive measurement of mnAb concentrations in source plasmas and drug product; these ELISAs are also faster and more accurate than cell-based assay.
Files in This Item
There are no files associated with this item.
Appears in
Collections
College of Life Sciences and Biotechnology > Division of Life Sciences > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Altmetrics

Total Views & Downloads

BROWSE