Transforming Growth Factor-beta 1-induced Human Subconjunctival Fibrosis is Mediated by MicroRNA 143/145 Expression
DC Field | Value | Language |
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dc.contributor.author | Hwang, Young Hoon | - |
dc.contributor.author | Jung, Sun-Ah | - |
dc.contributor.author | Lyu, Jungmook | - |
dc.contributor.author | Kim, Yong Yeon | - |
dc.contributor.author | Lee, Joon H. | - |
dc.date.accessioned | 2021-09-01T15:52:24Z | - |
dc.date.available | 2021-09-01T15:52:24Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2019-05 | - |
dc.identifier.issn | 0146-0404 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/65927 | - |
dc.description.abstract | PURPOSE. To investigate the roles and pathways of microRNAs 143 and 145 in transforming growth factor (TGF)-beta 1-induced human subconjunctival fibrosis. METHODS. Human tenon's capsule fibroblasts (HTFs) were obtained from a healthy eye. After treating cultured HTFs with TGF-beta 1, the expression of microRNAs 143 and 145 was evaluated using polymerase chain reaction. To identify the pathways of TGF-beta 1-induced microRNA 143/145 expression, HTFs were treated with specific inhibitors of p38MAPK, PI3K/Akt, JNK, ERK, and with siRNAs for SMAD2 and SMAD4. Mutagenesis studies were performed to evaluate the role of the CArG box and SMAD-binding element (SBE). To investigate the role of microRNA 143/145 in TGF-beta 1-induced myofibroblast transdifferentiation, microRNA 143/145 mimics and microRNA 143/145 inhibitors were applied to the HTFs. RESULTS. Array analysis revealed that TGF-beta 1 induced the expression of microRNA 143/145 in a dose-and time-dependent manner. When inhibitors and siRNAs for p38MAPK, PI3K/Akt, ERK, and JNK were applied, the TGF-beta 1-induced expression of microRNA 143/145 was inhibited; however, SMAD2 and SMAD4 inhibition did not affect the TGF-beta 1-induced expression of these microRNAs. In the mutagenesis studies, both the CArG box and SBE were associated with TGF-b1-induced expression of microRNA 143/145. Mimics of microRNA 143/145 induced increased myofibroblast formation, whereas their inhibitors had the opposite effect. CONCLUSIONS. TGF-beta 1-induced human subconjunctival fibrosis was mediated by the expression of microRNA 143/145, mainly via SMAD-independent pathways. Inhibition of TGF-beta 1-induced microRNA 143/145 expression in HTFs might represent a novel strategy to prevent subconjunctival fibrosis. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ASSOC RESEARCH VISION OPHTHALMOLOGY INC | - |
dc.subject | CAPSULE FIBROBLAST PROLIFERATION | - |
dc.subject | I COLLAGEN EXPRESSION | - |
dc.subject | SERUM RESPONSE FACTOR | - |
dc.subject | TENONS CAPSULE | - |
dc.subject | TGF-BETA | - |
dc.subject | SP1 PATHWAY | - |
dc.subject | GLAUCOMA | - |
dc.subject | SUPPRESSION | - |
dc.subject | MIR-29B | - |
dc.subject | TRABECULECTOMY | - |
dc.title | Transforming Growth Factor-beta 1-induced Human Subconjunctival Fibrosis is Mediated by MicroRNA 143/145 Expression | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Yong Yeon | - |
dc.identifier.doi | 10.1167/iovs.19-26797 | - |
dc.identifier.scopusid | 2-s2.0-85066061223 | - |
dc.identifier.wosid | 000468980600029 | - |
dc.identifier.bibliographicCitation | INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, v.60, no.6, pp.2064 - 2071 | - |
dc.relation.isPartOf | INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE | - |
dc.citation.title | INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE | - |
dc.citation.volume | 60 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 2064 | - |
dc.citation.endPage | 2071 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Ophthalmology | - |
dc.relation.journalWebOfScienceCategory | Ophthalmology | - |
dc.subject.keywordPlus | CAPSULE FIBROBLAST PROLIFERATION | - |
dc.subject.keywordPlus | I COLLAGEN EXPRESSION | - |
dc.subject.keywordPlus | SERUM RESPONSE FACTOR | - |
dc.subject.keywordPlus | TENONS CAPSULE | - |
dc.subject.keywordPlus | TGF-BETA | - |
dc.subject.keywordPlus | SP1 PATHWAY | - |
dc.subject.keywordPlus | GLAUCOMA | - |
dc.subject.keywordPlus | SUPPRESSION | - |
dc.subject.keywordPlus | MIR-29B | - |
dc.subject.keywordPlus | TRABECULECTOMY | - |
dc.subject.keywordAuthor | glaucoma | - |
dc.subject.keywordAuthor | fibrosis | - |
dc.subject.keywordAuthor | TGF-beta | - |
dc.subject.keywordAuthor | microRNA | - |
dc.subject.keywordAuthor | conjunctiva | - |
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