NIH3T3 Directs Memory-Fated CTL Programming and Represses High Expression of PD-1 on Antitumor CTLs
- Authors
- Qin, Yingyu; Lee, Yuna; Seo, Jaeho; Kim, Taehyun; Shin, Jung Hoon; Park, Se-Ho
- Issue Date
- 11-4월-2019
- Publisher
- FRONTIERS MEDIA SA
- Keywords
- NIH3T3-CM; cytotoxic T lymphocytes; memory precursor; memory CD8(+) T cells; adoptive cell therapy
- Citation
- FRONTIERS IN IMMUNOLOGY, v.10
- Indexed
- SCIE
SCOPUS
- Journal Title
- FRONTIERS IN IMMUNOLOGY
- Volume
- 10
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/66025
- DOI
- 10.3389/fimmu.2019.00761
- ISSN
- 1664-3224
- Abstract
- Memory CD8(+) T cells have long been considered a promising population for adoptive cell therapy (ACT) due to their long-term persistence and robust re-stimulatory response. NIH3T3 is an immortalized mouse embryonic fibroblast cell line. We report that NIH3T3-conditioned medium (CM) can augment effector functions of CTLs following antigen priming and confer phenotypic and transcriptional properties of central memory cells. After NIH3T3-CM-educated CTLs were infused into naive mice, they predominantly developed to central memory cells. A large number of NIH3T3-CM-educated CTLs with high functionality persisted and infiltrated to tumor mass. In addition, NIH3T3-CM inhibited CTLs expression of PD-1 in vitro and repressed their high expression of PD-1 in tumor microenvironment after adoptive transfer. Consequently, established tumor models showed that infusion of NIH3T3-CM-educated CTLs dramatically improved CTL mediated-antitumor immunity. Furthermore, NIH3T3-CM also promoted human CD8(+) T cells differentiation into memory cells. These results suggest that NIH3T3-CM-programmed CTLs are good candidates for adoptive transfer in tumor therapy.
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Collections - Graduate School > Department of Life Sciences > 1. Journal Articles
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