Electrical impulse effects on degenerative human annulus fibrosus model to reduce disc pain using micro-electrical impulse-on-a-chip
DC Field | Value | Language |
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dc.contributor.author | Shin, JaeHee | - |
dc.contributor.author | Hwang, MinHo | - |
dc.contributor.author | Back, SeungMin | - |
dc.contributor.author | Nam, HyoGeun | - |
dc.contributor.author | Yoo, ChangMin | - |
dc.contributor.author | Park, JeongHun | - |
dc.contributor.author | Son, HyeongGuk | - |
dc.contributor.author | Lee, JaeWon | - |
dc.contributor.author | Lim, HyunJung | - |
dc.contributor.author | Lee, KwangHo | - |
dc.contributor.author | Moon, HongJoo | - |
dc.contributor.author | Kim, JooHan | - |
dc.contributor.author | Cho, HanSang | - |
dc.contributor.author | Choi, Hyuk | - |
dc.date.accessioned | 2021-09-01T16:14:03Z | - |
dc.date.available | 2021-09-01T16:14:03Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2019-04-09 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/66033 | - |
dc.description.abstract | Electrical stimulation of cells and tissues for therapeutic benefit is a well-established method. Although animal studies can emulate the complexity of an organism's physiology, lab-on-a-chip platforms provide a suitable primary model for follow-up animal studies. Thus, inexpensive and easy-to-use platforms for in vitro human cell studies are required. In the present study, we designed a microelectrical impulse (micro-EI)-on-a-chip (micro-EI-chip), which can precisely control electron density and adjust the frequency based on a micro-EI. The micro-EI-chip can stimulate cells at various micro-EI densities (0-500 mV/mm) and frequencies (0-300 Hz), which enables multiple co-culture of different cell types with or without electrical stimulation. As a proof-of-concept study, a model involving degenerative inflamed human annulus fibrosus (hAF) cells was established in vitro and the effects of micro-EI on inflamed hAF cells were evaluated using the micro-EI-chip. Stimulation of the cells (150 mV/mm at 200 Hz) inhibited the secretion of inflammatory cytokines and downregulated the activities of extracellular matrix-modifying enzymes and matrix metalloproteinase-1. These results show that micro-EI stimulation could affect degenerative diseases based on inflammation, implicating the micro-EI-chip as being useful for basic research of electroceuticals. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.subject | NUCLEUS PULPOSUS CELLS | - |
dc.subject | TUMOR-NECROSIS-FACTOR | - |
dc.subject | NERVE GROWTH-FACTOR | - |
dc.subject | CYTOKINE PRODUCTION | - |
dc.subject | THP-1 CELLS | - |
dc.subject | STIMULATION | - |
dc.subject | EXPRESSION | - |
dc.subject | INFLAMMATION | - |
dc.subject | INDUCTION | - |
dc.subject | RESPONSES | - |
dc.title | Electrical impulse effects on degenerative human annulus fibrosus model to reduce disc pain using micro-electrical impulse-on-a-chip | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Hwang, MinHo | - |
dc.contributor.affiliatedAuthor | Kim, JooHan | - |
dc.contributor.affiliatedAuthor | Choi, Hyuk | - |
dc.identifier.doi | 10.1038/s41598-019-42320-9 | - |
dc.identifier.scopusid | 2-s2.0-85064091898 | - |
dc.identifier.wosid | 000463869800029 | - |
dc.identifier.bibliographicCitation | SCIENTIFIC REPORTS, v.9 | - |
dc.relation.isPartOf | SCIENTIFIC REPORTS | - |
dc.citation.title | SCIENTIFIC REPORTS | - |
dc.citation.volume | 9 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.subject.keywordPlus | NUCLEUS PULPOSUS CELLS | - |
dc.subject.keywordPlus | TUMOR-NECROSIS-FACTOR | - |
dc.subject.keywordPlus | NERVE GROWTH-FACTOR | - |
dc.subject.keywordPlus | CYTOKINE PRODUCTION | - |
dc.subject.keywordPlus | THP-1 CELLS | - |
dc.subject.keywordPlus | STIMULATION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | INFLAMMATION | - |
dc.subject.keywordPlus | INDUCTION | - |
dc.subject.keywordPlus | RESPONSES | - |
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