Detailed Information

Cited 0 time in webofscience Cited 0 time in scopus
Metadata Downloads

Noninvasive Serum Metabolomic Profiling Reveals Elevated Kynurenine Pathway's Metabolites in Humans with Prostate Cancer

Authors
Khan, AdnanChoi, Soo AnNa, JinhyukPamungkas, Aryo DimasJung, Keum JiJee, Sun HaPark, Youngja H.
Issue Date
4월-2019
Publisher
AMER CHEMICAL SOC
Keywords
prostate cancer; liquid chromatography; metabolomics; biomarker; kynurenine
Citation
JOURNAL OF PROTEOME RESEARCH, v.18, no.4, pp.1532 - 1541
Indexed
SCIE
SCOPUS
Journal Title
JOURNAL OF PROTEOME RESEARCH
Volume
18
Number
4
Start Page
1532
End Page
1541
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/66472
DOI
10.1021/acs.jproteome.8b00803
ISSN
1535-3893
Abstract
This study aimed to apply high-resolution metabolomics to detect compounds that may contribute significantly to prostate cancer (PCa) development. The test population's sera for evaluating the metabolic differences consisted of healthy control (n = 96) and PCa (n = 50) groups. PCa patients were further divided into two groups based on whether their PSA level was >4 (n = 25) or <4 (n = 25). Univariate analysis was performed with the false discovery rate (FDR) at q = 0.05 to determine significantly different metabolites. Principal component analysis (PCA) and hierarchical clustering analysis (HCA) clearly distinguished healthy subjects from PCa groups, while no significant difference was observed in PCa patients with PSA level < 4 or > 4. Mummichog, in combination with the KEGG and MetaboAnalyst, showed that tryptophan metabolism along the kynurenine pathway was most significantly enriched, with log (p) < 0.05. L-Tryptophan, kynurenine, anthranilate, isophenoxazine, glutaryl-CoA, (S)-3-hydroxybutanoyl-CoA, acetoacetyl-CoA, and acetyl-CoA were upregulated in correlation with the PSA level of PCa patients; in contrast, indoxyl, indolelactate, and indole-3-ethanol, involved in the alternative pathway, were downregulated in the PCa patients. Validation and quantification of potential metabolites by MS/MS further confirmed the disruption of tryptophan, kynurenine, and anthranilate, suggesting that the metabolites of this pathway are potential biomarkers in patients with PCa.
Files in This Item
There are no files associated with this item.
Appears in
Collections
College of Pharmacy > Department of Pharmaceutical Science > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Altmetrics

Total Views & Downloads

BROWSE