Collagen Type I Containing Hybrid Hydrogel Enhances Cardiomyocyte Maturation in a 3D Cardiac Model
- Authors
- Edalat, Sam G.; Jang, Yongjun; Kim, Jongseong; Park, Yongdoo
- Issue Date
- 4월-2019
- Publisher
- MDPI
- Keywords
- matrigel-based hydrogel; embryonic stem-cell; cardiomyocyte maturation; collagen type I; TGF-1; FGF-4
- Citation
- POLYMERS, v.11, no.4
- Indexed
- SCIE
SCOPUS
- Journal Title
- POLYMERS
- Volume
- 11
- Number
- 4
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/66489
- DOI
- 10.3390/polym11040687
- ISSN
- 2073-4360
- Abstract
- In vitro maturation of cardiomyocytes in 3D is essential for the development of viable cardiac models for therapeutic and developmental studies. The method by which cardiomyocytes undergoes maturation has significant implications for understanding cardiomyocytes biology. The regulation of the extracellular matrix (ECM) by changing the composition and stiffness is quintessential for engineering a suitable environment for cardiomyocytes maturation. In this paper, we demonstrate that collagen type I, a component of the ECM, plays a crucial role in the maturation of cardiomyocytes. To this end, embryonic stem-cell derived cardiomyocytes were incorporated into Matrigel-based hydrogels with varying collagen type I concentrations of 0 mg, 3 mg, and 6 mg. Each hydrogel was analyzed by measuring the degree of stiffness, the expression levels of MLC2v, TBX18, and pre-miR-21, and the size of the hydrogels. It was shown that among the hydrogel variants, the Matrigel-based hydrogel with 3 mg of collagen type I facilitates cardiomyocyte maturation by increasing MLC2v expression. The treatment of transforming growth factor 1 (TGF-1) or fibroblast growth factor 4 (FGF-4) on the hydrogels further enhanced the MLC2v expression and thereby cardiomyocyte maturation.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - Graduate School > Department of Biomedical Sciences > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.