Farnesylation-defective Rheb Increases Axonal Length Independently of mTORC1 Activity in Embryonic Primary Neurons
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Choi, Seunghyuk | - |
dc.contributor.author | Sadra, Ali | - |
dc.contributor.author | Kang, Jieun | - |
dc.contributor.author | Ryu, Jae Ryun | - |
dc.contributor.author | Kim, June Hoan | - |
dc.contributor.author | Sun, Woong | - |
dc.contributor.author | Huh, Sung-Oh | - |
dc.date.accessioned | 2021-09-01T17:04:45Z | - |
dc.date.available | 2021-09-01T17:04:45Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2019-04 | - |
dc.identifier.issn | 1226-2560 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/66530 | - |
dc.description.abstract | Rheb (Ras homolog enriched in the brain) is a small GTPase protein that plays an important role in cell signaling for development of the neocortex through modulation of mTORC1 (mammalian-target-of-rapamycin-complex- 1) activity. mTORC1 is known to control various biological processes including axonal growth in forming complexes at the lysosomal membrane compartment. As such, anchoring of Rheb on the lysosomal membrane via the farnesylation of Rheb at its cysteine residue (C180) is required for its promotion of mTOR activity To test the significance of Rheb farnesylation, we overexpressed a farnesylation mutant form of Rheb, Rheb C180S, in primary rat hippocampal neurons and also in mouse embryonic neurons using in utero electroporation. Interestingly, we found that Rheb C180S maintained promotional effect of axonal elongation similar to the wild-type Rheb in both test systems. On the other hand, Rheb C180S failed to exhibit the multiple axon-promoting effect which is found in wild-type Rheb. The levels of phospho-4EBP1, a downstream target of mTORC1, were surprisingly increased in Rheb C180S transfected neurons, despite the levels of phosphorvlated mTOR being significantly decreased compared to control vector transfectants. A specific mTORC1 inhibitor, rapamycin, also could not completely abolish axon elongation characteristics of Rheb C180S in transfected cells. Our data suggests that Rheb in a non-membrane compartment can promote the axonal elongation via phosphorylation of 4EBP1 and through an mTORC1-independent pathway. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | KOREAN SOC BRAIN & NEURAL SCIENCE, KOREAN SOC NEURODEGENERATIVE DISEASE | - |
dc.subject | TUBEROUS SCLEROSIS COMPLEX | - |
dc.subject | TARGET | - |
dc.subject | LOCALIZATION | - |
dc.subject | CYTOSKELETON | - |
dc.subject | MIGRATION | - |
dc.subject | POLARITY | - |
dc.subject | TSC2 | - |
dc.title | Farnesylation-defective Rheb Increases Axonal Length Independently of mTORC1 Activity in Embryonic Primary Neurons | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, June Hoan | - |
dc.contributor.affiliatedAuthor | Sun, Woong | - |
dc.identifier.doi | 10.5607/en.2019.28.2.172 | - |
dc.identifier.scopusid | 2-s2.0-85066908355 | - |
dc.identifier.wosid | 000468151200005 | - |
dc.identifier.bibliographicCitation | EXPERIMENTAL NEUROBIOLOGY, v.28, no.2, pp.172 - 182 | - |
dc.relation.isPartOf | EXPERIMENTAL NEUROBIOLOGY | - |
dc.citation.title | EXPERIMENTAL NEUROBIOLOGY | - |
dc.citation.volume | 28 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 172 | - |
dc.citation.endPage | 182 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART002464008 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalResearchArea | Neurosciences & Neurology | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.relation.journalWebOfScienceCategory | Neurosciences | - |
dc.subject.keywordPlus | TUBEROUS SCLEROSIS COMPLEX | - |
dc.subject.keywordPlus | TARGET | - |
dc.subject.keywordPlus | LOCALIZATION | - |
dc.subject.keywordPlus | CYTOSKELETON | - |
dc.subject.keywordPlus | MIGRATION | - |
dc.subject.keywordPlus | POLARITY | - |
dc.subject.keywordPlus | TSC2 | - |
dc.subject.keywordAuthor | Axons | - |
dc.subject.keywordAuthor | mTOR protein | - |
dc.subject.keywordAuthor | Rheb protein | - |
dc.subject.keywordAuthor | Protein farnesylation | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.