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Metabolic engineering of Enterobacter aerogenes to improve the production of 2,3-butanediol

Authors
Thapa, Laxmi PrasadLee, Sang JunPark, ChulhwanKim, Seung Wook
Issue Date
15-3월-2019
Publisher
ELSEVIER SCIENCE BV
Keywords
2,3-Butanediol; Enterobacter aerogenes; Biorefinery; Metabolic engineering
Citation
BIOCHEMICAL ENGINEERING JOURNAL, v.143, pp.169 - 178
Indexed
SCIE
SCOPUS
Journal Title
BIOCHEMICAL ENGINEERING JOURNAL
Volume
143
Start Page
169
End Page
178
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/66654
DOI
10.1016/j.bej.2018.12.019
ISSN
1369-703X
Abstract
The enhanced production of 2,3-butanediol was investigated using a metabolic engineering approach and optimized fermentation conditions. New engineered strains of Enterobacter aerogenes ATCC 29007 were developed by deleting the D-lactate dehydrogenase (ldhA), phosphate acetyltransferase (pta), malate dehydrogenase (mdh), and acetaldehyde dehydrogenase (acdh) genes to block the production of lactate, acetate, succinate, and ethanol, respectively. The resulting engineered strain E. aerogenes SUMI02 (Delta ldhA Delta pta) produced 36.5 g/L of 2,3-butanediol in flask cultivation, an amount 8.11 times greater than that of its wild type counterpart (4.5 g/L). In addition, the 2,3-butanediol production and productivity reached 38.24 g/L and 0.8 g/L/h, respectively, in the batch fermentation using a bioreactor.
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