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Microfluidic Fabrication of Encoded Hydrogel Microparticles for Application in Multiplex Immunoassay

Authors
Roh, Yoon HoLee, Hyun JeeBong, Ki Wan
Issue Date
3월-2019
Publisher
KOREAN BIOCHIP SOCIETY-KBCS
Keywords
Multiplex immunoassay; protein detection; suspension array; bead-based array; hydrogel; microparticle
Citation
BIOCHIP JOURNAL, v.13, no.1, pp.64 - 81
Indexed
SCIE
SCOPUS
KCI
Journal Title
BIOCHIP JOURNAL
Volume
13
Number
1
Start Page
64
End Page
81
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/67054
DOI
10.1007/s13206-019-3104-z
ISSN
1976-0280
Abstract
Recent interests in comprehensive protein surveys and protein biomarker studies have led to an increased demand for simultaneous measurement of multiple proteins in a single sample. Among various multiplex techniques, bead-based immunoassays, which use encoded particles attached with capture probes, have demonstrated distinct advantages of fluid-phase kinetics, high precision, and flexible target selection. In particular, encoded hydrogel particles composed of porous, hydrophilic, three-dimensional polymers have received positive attention because they enhance the binding kinetics of proteins, reduce protein denaturation, and increase the loading density of capture probes. Microfluidic techniques have been extensively used to fabricate the encoded hydrogel particles for multiplex immunoassays, enabling mass-production of highly monodisperse particles with complex morphologies in mild synthesis conditions. In this paper, we review microfluidic techniques available for the synthesis of encoded hydrogel particles and the important design parameters that determine the particles' immunoassay performance. We also discuss currently reported multiplex immunoassay platforms that are based on encoded hydrogel particles.
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