Intestinal barrier disruption and dysregulated mucosal immunity contribute to kidney fibrosis in chronic kidney disease
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yang, Jihyun | - |
dc.contributor.author | Lim, Sung Yoon | - |
dc.contributor.author | Ko, Yoon Sook | - |
dc.contributor.author | Lee, Hee Young | - |
dc.contributor.author | Oh, Se Won | - |
dc.contributor.author | Kim, Myung Gyu | - |
dc.contributor.author | Cho, Won Yong | - |
dc.contributor.author | Jo, Sang Kyung | - |
dc.date.accessioned | 2021-09-01T17:57:03Z | - |
dc.date.available | 2021-09-01T17:57:03Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2019-03 | - |
dc.identifier.issn | 0931-0509 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/67059 | - |
dc.description.abstract | Background Emerging evidence suggests that intestinal dysbiosis is associated with diverse pathological processes. In this study we demonstrated intestinal barrier disruption and aberrant mucosal immunity in 5/6 nephrectomized mice and the effect of probiotics on chronic kidney disease (CKD). Methods CKD was induced in 6-week-old mice by 5/6 nephrectomy. They were fed a lactobacilli mixture for 8weeks. Serum, urine and stool samples were collected for renal function assessments and gut microbiome analyses. Gut permeability, colon heat shock protein 70 (HSP70) and colon epithelial integrity were evaluated and cytokine levels in colon and kidney were measured. Colon leukocytes were analyzed by flow cytometry and bone marrow-derived cells were cocultured with lactobacilli mixture. Results In CKD mice, leaky gut' was accompanied by decreased colon HSP70 and claudin-1 expression, whereas it increased pore-forming claudin-2 expression and apoptosis. Although the percentage of regulatory T cells did not differ between CKD and control mice, cytokine expression and the ratio of CX(3)CR1intermediate:CX(3)CR1high pro-inflammatory/resident macrophages increased in the colon of CKD mice. Orally administered lactobacilli partially mitigated the CKD-induced leaky gut'; restored colon epithelial HSP70, claudin-1 and claudin-2 expression and decreased apoptosis. Probiotic treatment also restored the CX(3)CR1intermediate:CX(3)CR1high macrophage ratio and increased circular dichroism (CD)103(+)CD11c(+) regulatory dendritic cells in the colon. These changes suppressed systemic inflammation and kidney fibrosis. Conclusions Our results suggest that intestinal dysbiosis-associated gut barrier disruption and aberrant mucosal immunity are important for the systemic inflammation and progressive fibrosis of CKD. Targeting the intestine might provide novel therapeutic opportunities for CKD. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | OXFORD UNIV PRESS | - |
dc.subject | CHRONIC HEART-FAILURE | - |
dc.subject | GUT MICROBIOTA | - |
dc.subject | TIGHT JUNCTION | - |
dc.subject | INFLAMMATION | - |
dc.subject | PROBIOTICS | - |
dc.subject | MONOCYTE | - |
dc.subject | CELLS | - |
dc.title | Intestinal barrier disruption and dysregulated mucosal immunity contribute to kidney fibrosis in chronic kidney disease | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Oh, Se Won | - |
dc.contributor.affiliatedAuthor | Kim, Myung Gyu | - |
dc.contributor.affiliatedAuthor | Cho, Won Yong | - |
dc.contributor.affiliatedAuthor | Jo, Sang Kyung | - |
dc.identifier.doi | 10.1093/ndt/gfy172 | - |
dc.identifier.scopusid | 2-s2.0-85061292388 | - |
dc.identifier.wosid | 000461162000010 | - |
dc.identifier.bibliographicCitation | NEPHROLOGY DIALYSIS TRANSPLANTATION, v.34, no.3, pp.419 - 428 | - |
dc.relation.isPartOf | NEPHROLOGY DIALYSIS TRANSPLANTATION | - |
dc.citation.title | NEPHROLOGY DIALYSIS TRANSPLANTATION | - |
dc.citation.volume | 34 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 419 | - |
dc.citation.endPage | 428 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Transplantation | - |
dc.relation.journalResearchArea | Urology & Nephrology | - |
dc.relation.journalWebOfScienceCategory | Transplantation | - |
dc.relation.journalWebOfScienceCategory | Urology & Nephrology | - |
dc.subject.keywordPlus | CHRONIC HEART-FAILURE | - |
dc.subject.keywordPlus | GUT MICROBIOTA | - |
dc.subject.keywordPlus | TIGHT JUNCTION | - |
dc.subject.keywordPlus | INFLAMMATION | - |
dc.subject.keywordPlus | PROBIOTICS | - |
dc.subject.keywordPlus | MONOCYTE | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordAuthor | chronic kidney disease | - |
dc.subject.keywordAuthor | dysbiosis | - |
dc.subject.keywordAuthor | inflammation | - |
dc.subject.keywordAuthor | macrophages | - |
dc.subject.keywordAuthor | probiotics | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.