Metformin-activated AMPK regulates beta-catenin to reduce cell proliferation in colon carcinoma RKO cells
DC Field | Value | Language |
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dc.contributor.author | Park, Song Yi | - |
dc.contributor.author | Kim, Dasarang | - |
dc.contributor.author | Kee, Sun-Ho | - |
dc.date.accessioned | 2021-09-01T18:03:50Z | - |
dc.date.available | 2021-09-01T18:03:50Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2019-03 | - |
dc.identifier.issn | 1792-1074 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/67110 | - |
dc.description.abstract | Metformin can suppress cell proliferation and viability by altering mitochondrial energy metabolism and by the activation of 5 '-adenosine monophosphate-activated protein kinase (AMPK). The current study demonstrated that metformin-induced suppression of cell proliferation is further potentiated by AMPK-mediated suppression of beta-catenin-dependent wingless-type (Wnt) signaling. Treatment with metformin reduced mitochondrial oxidative phosphorylation and glycolysis, leading to an energy imbalance that may induce AMPK phosphorylation in RKO cells. Metformin treatment also decreased beta-catenin expression in the cytoplasm and nucleus. Active AMPK was revealed to be associated with beta-catenin. The decrease in beta-catenin expression was inhibited by proteosome inhibition through phosphorylation of beta-catenin at serine 33/37. Given that nuclear translocation-associated phosphorylation of beta-catenin at serine was maintained, the association of beta-catenin with AMPK may sequester beta-catenin in the cytoplasm and lead to proteosomal degradation. Furthermore, metformin-induced suppression of cell proliferation was partially recovered by AMPK inhibition, while metformin inhibited Wnt-mediated cell proliferation and beta-catenin expression. The present results suggest that AMPK activation can suppress beta-catenin-dependent Wnt signaling by cytoplasmic sequestering of beta-catenin through AMPK, which further decreases cell proliferation in addition to metformin-induced mitochondrial dysfunction. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | SPANDIDOS PUBL LTD | - |
dc.subject | EPITHELIAL-MESENCHYMAL TRANSITION | - |
dc.subject | PROTEIN-KINASE | - |
dc.subject | CANCER PREVENTION | - |
dc.subject | TARGETING AMPK | - |
dc.subject | RISK | - |
dc.subject | MECHANISMS | - |
dc.subject | COMPLEX | - |
dc.subject | MTOR | - |
dc.subject | LKB1 | - |
dc.title | Metformin-activated AMPK regulates beta-catenin to reduce cell proliferation in colon carcinoma RKO cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kee, Sun-Ho | - |
dc.identifier.doi | 10.3892/ol.2019.9892 | - |
dc.identifier.scopusid | 2-s2.0-85062149205 | - |
dc.identifier.wosid | 000460555900016 | - |
dc.identifier.bibliographicCitation | ONCOLOGY LETTERS, v.17, no.3, pp.2695 - 2702 | - |
dc.relation.isPartOf | ONCOLOGY LETTERS | - |
dc.citation.title | ONCOLOGY LETTERS | - |
dc.citation.volume | 17 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 2695 | - |
dc.citation.endPage | 2702 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Oncology | - |
dc.relation.journalWebOfScienceCategory | Oncology | - |
dc.subject.keywordPlus | EPITHELIAL-MESENCHYMAL TRANSITION | - |
dc.subject.keywordPlus | PROTEIN-KINASE | - |
dc.subject.keywordPlus | CANCER PREVENTION | - |
dc.subject.keywordPlus | TARGETING AMPK | - |
dc.subject.keywordPlus | RISK | - |
dc.subject.keywordPlus | MECHANISMS | - |
dc.subject.keywordPlus | COMPLEX | - |
dc.subject.keywordPlus | MTOR | - |
dc.subject.keywordPlus | LKB1 | - |
dc.subject.keywordAuthor | 5 &apos | - |
dc.subject.keywordAuthor | -adenosine monophosphate-activated protein kinase | - |
dc.subject.keywordAuthor | beta-catenin | - |
dc.subject.keywordAuthor | metformin | - |
dc.subject.keywordAuthor | adenosine 5 &apos | - |
dc.subject.keywordAuthor | -triphosphate production | - |
dc.subject.keywordAuthor | cell proliferation | - |
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