A dual role of TGF-beta in human osteoclast differentiation mediated by Smad1 versus Smad3 signaling
DC Field | Value | Language |
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dc.contributor.author | Lee, Bitnara | - |
dc.contributor.author | Oh, Younseo | - |
dc.contributor.author | Jo, Sungsin | - |
dc.contributor.author | Kim, Tae-Hwan | - |
dc.contributor.author | Ji, Jong Dae | - |
dc.date.accessioned | 2021-09-01T19:40:39Z | - |
dc.date.available | 2021-09-01T19:40:39Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2019-02 | - |
dc.identifier.issn | 0165-2478 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/67740 | - |
dc.description.abstract | TGF-beta 1 is highly expressed in the synovial tissue of patients with rheumatoid arthritis and is known as a cytokine that plays an important role in tissue repair and immune cell regulation. However, the role of TGF-beta 1 is still unclear in osteoclastogenesis. In this study, we examined the effect of TGF-beta 11 on osteoclast differentiation and the underlying mechanism using healthy human peripheral blood monocytes. TGF-beta 1 was found to inhibit osteoclast differentiation and decrease the expression of osteoclast-specific genes such as acid phosphatase 5, tartrate resistant and cathepsin K. Levels of NFAT1, an important transcription factor in osteoclastogenesis, were also reduced. In addition, TGF-beta 11 suppressed receptor activator of NF-x13 (RANK) ligand-induced NF-kappa B and p38 MAPK signaling. Inhibition of osteoclast differentiation by TGF-beta 1 was reversed by 1 pM SB431542 (an inhibitor of ALK4/5/7), which inhibited TGF-beta 1-induced phosphorylation of SMAD1, but not that of SMAD3. TGF-beta 1 also restricted RANK expression, and this was partially reversed by 1 pM SB431542. In contrast, the inhibition of SMAD3 by SIS3 (an inhibitor of SMAD3) reduced the osteoclast formation. TGF-f31 has both inhibitory and stimulatory effects on human osteoclast differentiation, and that these opposing functions are mediated by SMAD1 and SMAD3 signaling, respectively. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.subject | EXPRESSION | - |
dc.subject | TGF-BETA-1 | - |
dc.subject | BONE | - |
dc.subject | RANK | - |
dc.subject | MATRIX-METALLOPROTEINASE-9 | - |
dc.title | A dual role of TGF-beta in human osteoclast differentiation mediated by Smad1 versus Smad3 signaling | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Ji, Jong Dae | - |
dc.identifier.doi | 10.1016/j.imlet.2018.12.003 | - |
dc.identifier.scopusid | 2-s2.0-85058409987 | - |
dc.identifier.wosid | 000460493300005 | - |
dc.identifier.bibliographicCitation | IMMUNOLOGY LETTERS, v.206, pp.33 - 40 | - |
dc.relation.isPartOf | IMMUNOLOGY LETTERS | - |
dc.citation.title | IMMUNOLOGY LETTERS | - |
dc.citation.volume | 206 | - |
dc.citation.startPage | 33 | - |
dc.citation.endPage | 40 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Immunology | - |
dc.relation.journalWebOfScienceCategory | Immunology | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | TGF-BETA-1 | - |
dc.subject.keywordPlus | BONE | - |
dc.subject.keywordPlus | RANK | - |
dc.subject.keywordPlus | MATRIX-METALLOPROTEINASE-9 | - |
dc.subject.keywordAuthor | Osteoclast | - |
dc.subject.keywordAuthor | TGF-beta | - |
dc.subject.keywordAuthor | Smad1 | - |
dc.subject.keywordAuthor | Smad3 | - |
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